CRTAP Polyclonal antibody

CRTAP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15724-1-AP

Synonyms

cartilage associated protein, CASP, CRTAP, LEPREL3

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Validation Data Gallery

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  WB analysis using 15724-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15724-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 15724-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 15724-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15724-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15724-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15724-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 15724-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 15724-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15724-1-AP

  IP result of anti-CRTAP (IP:15724-1-AP, 4ug; Detection:15724-1-AP 1:500) with HEK-293 cells lysate 3000 ug.

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  IHC staining of human kidney using 15724-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15724-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15724-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CRTAP antibody (15724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue).

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Tested Applications

• Positive WB detected in: HT-1080 cells, human stomach tissue, NIH/3T3 cells, ROS1728 cells, HEK-293 cells, HeLa cells, human colon tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

15724-1-AP targets CRTAP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRTAP fusion protein Ag8459
• Full Name: cartilage associated protein
• Calculated Molecular Weight: 46.5 kDa
• Observed Molecular Weight: 43-47 kDa
• GenBank Accession Number: BC008745
• Gene Symbol: CRTAP
• Gene ID (NCBI): 10491
• RRID: AB_2261089
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Med Oncol; Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer; Authors - Liangshun Zhang

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