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  • KD/KO Validated

CRTC2,TORC2 Polyclonal antibody

CRTC2,TORC2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12497-1-AP

Synonyms

CRTC2, RP11 422P24.6, TORC 2, TORC2, Transducer of CREB protein 2



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, mouse liver tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:500
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12497-1-AP targets CRTC2,TORC2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity bovine, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRTC2,TORC2 fusion protein Ag3167
Full Name CREB regulated transcription coactivator 2
Calculated molecular weight 693 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC053562
Gene symbol CRTC2
Gene ID (NCBI) 200186
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .

Protocols

Product Specific Protocols
WB protocol for CRTC2,TORC2 antibody 12497-1-APDownload protocol
IHC protocol for CRTC2,TORC2 antibody 12497-1-APDownload protocol
IF protocol for CRTC2,TORC2 antibody 12497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

BMC Complement Med Ther

Zhenqing recipe attenuates non-alcoholic fatty liver disease by regulating the SIK1/CRTC2 signaling in experimental diabetic rats.

Authors - Daofei Song
ratWB

Curr Med Sci

Berberine Inhibits Gluconeogenesis in Skeletal Muscles and Adipose Tissues in Streptozotocin-induced Diabetic Rats via LKB1-AMPK-TORC2 Signaling Pathway.

Authors - Xiao-Hu Xu
mouseIF

Nat Metab

In vivo screen identifies a SIK inhibitor that induces β cell proliferation through a transient UPR.

Authors - Jérémie Charbord
ratWB

PLoS One

Adenovirus-mediated expression of SIK1 improves hepatic glucose and lipid metabolism in type 2 diabetes mellitus rats.

Authors - DaoFei Song
humanWB,IF

Front Endocrinol (Lausanne)

SIK1 Regulates CRTC2-Mediated Gluconeogenesis Signaling Pathway in Human and Mouse Liver Cells.

Authors - Chang Wang
ratIF

Am J Physiol Cell Physiol

CRTC2 activation in the suprachiasmatic nucleus, but not paraventricular nucleus, varies in a diurnal fashion and increases with night-time light exposure.

Authors - Julie Highland