Cryptochrome 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cryptochrome 2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

29

Reviews (2)

Cat no : 13997-1-AP

Synonyms

CRY2, Cryptochrome 2, HCRY2, KIAA0658, PHLL2

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Validation Data Gallery

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  WB analysis using 13997-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13997-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13997-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13997-1-AP

  IP result of anti-Cryptochrome 2 (IP:13997-1-AP, 4ug; Detection:13997-1-AP 1:300) with mouse brain tissue lysate 5200ug.

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  IHC staining of mouse brain using 13997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 13997-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, Y79 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 28 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

13997-1-AP targets Cryptochrome 2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cryptochrome 2 fusion protein Ag5085
• Full Name: cryptochrome 2 (photolyase-like)
• Calculated Molecular Weight: 593 aa, 67 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC041814
• Gene Symbol: CRY2
• Gene ID (NCBI): 1408
• RRID: AB_10860100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cryptochrome circadian clock 2 (CRY2) is a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. Loss of CRY2 stabilizes c-MYC and enhances cellular transformation. CRY2 can function as a co-factor for the SCF substrate adaptor FBXL3 (PMID: 27840026). CRY2 has two isoform with MW of 60 and 67 kDa. This antibody can recognize CRY1 and CRY2 due to the high homology.

Publications

Species	Application	Title
human	WB	Sci Adv; Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle.; Authors - Yool Lee
rat	WB	Cell Death Differ; CircRNA-vgll3 promotes osteogenic differentiation of adipose-derived mesenchymal stem cells via modulating miRNA-dependent integrin α5 expression.; Authors - Dandan Zhang
mouse	WB	Curr Biol; Cryptochromes-Mediated Inhibition of the CRL4Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism.; Authors - Luca Rizzini
human	WB	Elife; KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.; Authors - Yool Lee
human,mouse	WB,IF	Mol Ther Nucleic Acids; Inhibition of CRY2 by STAT3/miRNA-7-5p Promotes Osteoblast Differentiation through Upregulation of CLOCK/BMAL1/P300 Expression.; Authors - Zhenghui Tang ; KD Validated
human,mouse	WB,CoIP	Biochem Pharmacol; CRY1/2 regulate rhythmic CYP2A5 in mouse liver through repression of E4BP4; Authors - Luomin Lin ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Priya (Verified Customer) (01-11-2023)

I have used this for human cardiomyocytes cell line and mice liver and kin tisssue

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin

FH

Julia (Verified Customer) (01-14-2022)

Gives one clear band with the expected size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: U2OS

Cryptochrome 2 Antibody Western Blot validation (1:800 dilution) in U2OS (Cat no:13997-1-AP)