Alpha B Crystallin Polyclonal antibody

• Featured Product
• KD/KO Validated

Alpha B Crystallin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 15808-1-AP

Synonyms

CRYAB, CRYA2, CRYA, Alpha-crystallin B chain, Alpha(B)-crystallin

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Validation Data Gallery

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  WB analysis using 15808-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15808-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 15808-1-AP

  IP result of anti-Alpha B Crystallin (IP:15808-1-AP, 4ug; Detection:15808-1-AP 1:500) with U2OS cells lysate 1800 ug.

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  IHC staining of mouse eye using 15808-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15808-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15808-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15808-1-AP (Alpha B Crystallin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 15808-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Alpha B Crystallin antibody (15808-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse heart tissue, U2OS cells, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IP detected in: U2OS cells
• Positive IHC detected in: mouse eye tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Product Information

15808-1-AP targets Alpha B Crystallin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, fish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha B Crystallin fusion protein Ag8543
• Full Name: crystallin, alpha B
• Calculated Molecular Weight: 175 aa, 20 kDa
• Observed Molecular Weight: 20-22 kDa
• GenBank Accession Number: BC007008
• Gene Symbol: Alpha B Crystallin
• Gene ID (NCBI): 1410
• RRID: AB_2292175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha B-crystallin, encoded by CRYAB gene, is multifunctional, serving as both a major structural protein in the lens and a small heat-shock protein in other tissues in mammals. Alpha B-crystallin may contribute to the transparency and refractive index of the lens. Single nucleotide polymorphisms (SNPs) in the promoter region of CRYAB gene have been associated with in multiple sclerosis. Mutations in the CRYAB gene cause distinct clinical phenotypes including isolated posterior polar cataract, myofibrillar myopathy, cardiomyopathy, or a multisystemic disorder combining all these features. Impairment of alpha-B crystallin dimerization may be relevant to the pathogenesis of these disorders.

Publications

Species	Application	Title
mouse	IF	Brief Bioinform; iSMNN: batch effect correction for single-cell RNA-seq data via iterative supervised mutual nearest neighbor refinement.; Authors - Yuchen Yang
human	WB	Oxid Med Cell Longev; Resveratrol Modulation of Protein Expression in parkin-Mutant Human Skin Fibroblasts: A Proteomic Approach.; Authors - Daniele Vergara
human,mouse	WB	Elife; Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.; Authors - Vasiliki-Ilya Gargareta
mouse	WB	Glia; Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome; Authors - Sophie B Siems
human	WB	Front Cell Dev Biol; A Novel Ferroptosis-Related Prognostic Signature Reveals Macrophage Infiltration and EMT Status in Bladder Cancer.; Authors - Yilin Yan ; KD Validated
fish	ELISA	Food Res Int; Inhibitory effect of edible natural compounds with di- and tri-carboxyl moiety on endogenous protease inducing disassembly and degradation of myofibrils from grass carp (Ctenopharyngodon idella).; Authors - Lihong Ge