CRYZL1 Polyclonal antibody

CRYZL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16894-1-AP

Synonyms

4P11, CRYZL1, Protein 4P11, QOH 1, Zeta crystallin homolog

---

Validation Data Gallery

• 

  WB analysis of COS-7 using 16894-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16894-1-AP (CRYZL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16894-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16894-1-AP (CRYZL1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human ovary tumor using 16894-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16894-1-AP (CRYZL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 16894-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16894-1-AP (CRYZL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: COS-7 cells, MCF-7 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16894-1-AP targets CRYZL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRYZL1 fusion protein Ag10419
• Full Name: crystallin, zeta (quinone reductase)-like 1
• Calculated Molecular Weight: 349 aa, 39 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: BC013155
• Gene Symbol: CRYZL1
• Gene ID (NCBI): 9946
• RRID: AB_2878329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.