CSF1R/CD115 Recombinant monoclonal antibody

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• CSF1R/CD115 Recombinant monoclonal antibody (84919-6-RR)

CSF1R/CD115 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84919-6-RR Copied!

Clone No. 242213G11

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

Csf1r, CD115, CSF 1R, CSF-1 receptor, Csfmr

• 

  WB analysis using 84919-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84919-6-RR (CSF1R/CD115 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse spleen using 84919-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84919-6-RR (M-CSF R/CD115 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse spleen using 84919-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84919-6-RR (M-CSF R/CD115 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 84919-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84919-6-RR against Mouse CSF1R/CD115 were performed. The affinity constant is below 1 pM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: J774A.1 cells, RAW 264.7 cells, mouse spleen tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84919-6-RR targets CSF1R/CD115 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1816

  Product name: Recombinant Mouse CSF1R protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 20-511 aa of NM_001037859.2

  Sequence: APVIEPSGPELVVEPGETVTLRCVSNGSVEWDGPISPYWTLDPESPGSTLTTRNATFKNTGTYRCTELEDPMAGSTTIHLYVKDPAHSWNLLAQEVTVVEGQEAVLPCLITDPALKDSVSLMREGGRQVLRKTVYFFSPWRGFIIRKAKVLDSNTYVCKTMVNGRESTSTGIWLKVNRVHPEPPQIKLEPSKLVRIRGEAAQIVCSATNAEVGFNVILKRGDTKLEIPLNSDFQDNYYKKVRALSLNAVDFQDAGIYSCVASNDVGTRTATMNFQVVESAYLNLTSEQSLLQEVSVGDSLILTVHADAYPSIQHYNWTYLGPFFEDQRKLEFITQRAIYRYTFKLFLNRVKASEAGQYFLMAQNKAGWNNLTFELTLRYPPEVSVTWMPVNGSDVLFCDVSGYPQPSVTWMECRGHTDRCDEAQALQVWNDTHPEVLSQKPFDKVIIQSQLPIGTLKHNMTYFCKTHNSVGNSSQYFRAVSLGQSKQLPDES

• Full Name: colony stimulating factor 1 receptor
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 110-170 kDa
• GenBank Accession Number: NM_001037859.2
• Gene Symbol: CSF1R/CD115
• Gene ID (NCBI): 12978
• RRID: AB_3743846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09581
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).

Publications

What published studies show

The CSF1R/CD115 Recombinant monoclonal antibody (242213G11), catalog number 84919-6-RR, has 1 citation to date. The cited publication appeared in Molecular Pharmaceutics (Impact Factor 4.9), published in 2026. The research focuses on chemo-immunotherapy against malignant pleural effusion, specifically investigating the inhibition of the OCT4/M-CSF signaling pathway using dihydroartemisinin-loaded ZIF-8 nanoparticles in a mouse model. The antibody was applied via IHC in this study.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Mol Pharm; Dihydroartemisinin-Loaded ZIF-8 Nanoparticles Elicit Chemo-Immunotherapy against Malignant Pleural Effusion via Inhibition of OCT4/M-CSF Signaling Pathway.; Authors - Hui You