Anti-Human CSF1R/CD115 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98379-3-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF1R/CD115 Rabbit RecAb (98379-3-RR, Clone:250001D7) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98379-3-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF1R/CD115 Rabbit RecAb (98379-3-RR, Clone: 250001D7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CSF1R/CD115 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98379-3-RR

Clone No.250001D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CSF1R, C FMS, CD115, CSF 1 R, CSF-1 receptor

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98379-3-RR targets CSF1R/CD115 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: colony stimulating factor 1 receptor
• Calculated Molecular Weight: 972 aa, 108 kDa
• GenBank Accession Number: BC047521
• Gene Symbol: CSF1R/CD115
• Gene ID (NCBI): 1436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07333
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

The CSF-1 receptor (CSF1R), also called CD115, is activated by the homodimeric growth factors colony-stimulating factor-1 (CSF-1) and interleukin-34 (IL-34). It plays important roles in development and in innate immunity by regulating the development of most tissue macrophages and osteoclasts, of Langerhans cells of the skin, of Paneth cells of the small intestine, and of brain microglia. It also regulates the differentiation of neural progenitor cells and controls functions of oocytes and trophoblastic cells in the female reproductive tract. Owing to this broad tissue expression pattern, it plays a central role in neoplastic, inflammatory, and neurological diseases.