GM-CSF Polyclonal antibody

GM-CSF Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17762-1-AP

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, Molgramostin, Sargramostim



Tested Applications

Positive WB detected inRAW 264.7 cells, RAW264.7 cells
Positive IHC detected inhuman lung tissue, human cervical cancer tissue, human tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17762-1-AP targets GM-CSF in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GM-CSF fusion protein Ag12142
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Calculated molecular weight 144 aa, 16 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC108724
Gene symbol CSF2
Gene ID (NCBI) 1437
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

Protocols

Product Specific Protocols
WB protocol for GM-CSF antibody 17762-1-APDownload protocol
IHC protocol for GM-CSF antibody 17762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Lett

HIF-1α mediates tumor-nerve interactions through the up-regulation of GM-CSF in pancreatic ductal adenocarcinoma.

Authors - Haotian Wang
rat

Transpl Immunol

Pre-transplant infusion of donor-derived dendritic cells maintained at the immature stage by sinomenine increases splenic Foxp3(+) Tregs in recipient rats after renal allotransplantation.

Authors - Lian Li
humanWB,IHC

Cancer Manag Res

Antitumor Efficacy of Oncolytic Herpes Virus Type 1 Armed with GM-CSF in Murine Uveal Melanoma Xenografts.

Authors - Sisi Liu
human

J Virol

RNA helicase A is an important host factor involved in dengue virus replication.

Authors - Yi Wang
humanWB,IHC

FEBS J

Myeloid-derived suppressor cells promote epithelial ovarian cancer cell stemness by inducing the CSF2/p-STAT3 signalling pathway.

Authors - Xiaofeng Li
  • KD Validated
mouseIF

J Am Soc Nephrol

Tubular GM-CSF Promotes Late MCP-1/CCR2-Mediated Fibrosis and Inflammation after Ischemia/Reperfusion Injury.

Authors - Leyuan Xu