Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody, PBS Only

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  Sandwich ELISA standard curve of MP00953-1

  Sandwich ELISA standard curve of MP00953-1, Human CSF2RA/CD116 Recombinant Matched Antibody Pair - PBS only. 98066-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02335. 84023-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00953-1

  Serum of six individual healthy human donors was measured. The CSF2RA/CD116 concentration of detected samples was determined to be 2.0 ng/mL with a range of 0.9-4.7 ng/mL.

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  Sample test of MP00953-1

  The mean CSF2RA/CD116 concentration was determined to be 84.1 ng/mL in THP-1 cell extract based on a 4.0 mg/mL extract load and 59.0 ng/mL in HL-60 cell extract based on a 5.6 mg/mL extract load.

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  FC experiment of human PBMCs using 98066-1-RR (same clone as 98066-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (98066-1-RR, Clone:241266B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), or 0.25 ug Isotype Control. Cells were then stained with APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC). Cells were not fixed. This data was developed using the same antibody clone with 98066-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98066-1-RR (same clone as 98066-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (98066-1-RR, Clone:241266B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98066-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98066-1-RR (same clone as 98066-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CSF2RA/CD116 Rabbit Recombinant Antibody (98066-1-RR, Clone:241266B11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), and 0.25 ug APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC), and 0.25 ug Isotype Control. Cells were not fixed. This data was developed using the same antibody clone with 98066-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00953-1

  Cytometric bead array standard curve of MP00953-1, CSF2RA/CD116 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98066-1-PBS. Detection antibody: 84023-1-PBS. Standard: RP02335. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 98066-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98066-1-RR against Human CSF2RA/CD116 were performed. The affinity constant is 1.52 nM.

CSF2RA/CD116 Uni-rAb^® Recombinant Antibody for FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 98066-1-PBS

Clone No.241266B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD116, 241266B11, GMCSFR, GM CSF R alpha, CSF2RA

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Product Information

98066-1-PBS targets CSF2RA/CD116 in FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
• Calculated Molecular Weight: 46 kDa
• GenBank Accession Number: BC002635
• Gene Symbol: CD116
• Gene ID (NCBI): 1438
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P15509
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD116, also named as CSF2RA CDw116 CSF2RAX CSF2RAY CSF2RX CSF2RY GM-CSF-R-alpha GMCSFR and GMR, is a low affinity receptor for granulocyte-macrophage colony-stimulating factor. CD116 transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.