IL-3RB/CD131 Polyclonal antibody

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• IL-3RB/CD131 Polyclonal antibody (27148-1-AP)

IL-3RB/CD131 Polyclonal Antibody for IHC, ELISA

Cat No. 27148-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

CSF2RB, IL-3RB, IL3RB, GM-CSF/IL-3/IL-5 receptor common beta subunit, CDw131

• 

  IHC staining of human tonsillitis using 27148-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27148-1-AP (IL-3RB/CD131 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 27148-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27148-1-AP (IL-3RB/CD131 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 27148-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27148-1-AP (IL-3RB/CD131 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 27148-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27148-1-AP (IL-3RB/CD131 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27148-1-AP targets IL-3RB/CD131 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25914

  Product name: Recombinant human CSF2RB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 17-300 aa of BC070085

  Sequence: WERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSL

• Full Name: colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
• Calculated Molecular Weight: 889 aa, 96 kDa
• GenBank Accession Number: BC070085
• Gene Symbol: CSF2RB
• Gene ID (NCBI): 1439
• RRID: AB_2880775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P32927
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The IL-3RB/CD131 Polyclonal antibody (Cat# 27148-1-AP) has 2 total citations. These appear in Frontiers in Oncology and eLife. The cited studies span two research fields: cancer biology (immune-related gene signatures in triple-negative breast cancer) and inflammatory bowel disease (CD131's role in macrophage infiltration during ulcerative colitis pathogenesis). Both citations used the antibody for IHC in human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Front Oncol; Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.; Authors - Jinguo Zhang
human	IHC	Elife; CD131 contributes to ulcerative colitis pathogenesis by promoting macrophage infiltration.; Authors - Zhiyuan Wu