CSGALNACT2 Polyclonal antibody

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• CSGALNACT2 Polyclonal antibody (17420-1-AP)

CSGALNACT2 Polyclonal Antibody for WB, ELISA

Cat No. 17420-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Beta4GalNAcT 2, Beta4GalNAcT-2, CHGN2, Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 2, Chondroitin sulfate N-acetylgalactosaminyltransferase 2

• 

  WB analysis of K-562 using 17420-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17420-1-AP (CSGALNACT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of RAW264.7 using 17420-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 17420-1-AP (CSGALNACT2 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, RAW264.7

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17420-1-AP targets CSGALNACT2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11436

  Product name: Recombinant human CSGALNACT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 195-542 aa of BC030268

  Sequence: DEDDEQEDEEGPLGEKLIFNENDFVEGYYRTERDKGTQYELFFKKADLTEYRHVTLFRPFGPLMKVKSEMIDITRSIINIIVPLAERTEAFVQFMQNFRDVCIHQDKKIHLTVVYFGKEGLSKVKSILESVTSESNFHNYTLVSLNEEFNRGRGLNVGARAWDKGEVLMFFCDVDIYFSAEFLNSCRLNAEPGKKVFYPVVFSLYNPAIVYANQEVPPPVEQQLVHKKDSGFWRDFGFGMTCQYRSDFLTIGGFDMEVKGWGGEDVHLYRKYLHGDLIVIRTPVPGLFHLWHEKRCADELTPEQYRMCIQSKAMNEASHSHLGMLVFREEIETHLHKQAYRTNSEAVG

• Full Name: chondroitin sulfate N-acetylgalactosaminyltransferase 2
• Calculated Molecular Weight: 542 aa, 63 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC030268
• Gene Symbol: CSGALNACT2
• Gene ID (NCBI): 55454
• RRID: AB_2083878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N6G5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSGALNACT2 (Chondroitin Sulfate N-Acetylgalactosaminyltransferase 2) is a glycosyltransferase localized to the Golgi membrane that catalyzes the transfer of N-acetylgalactosamine (GalNAc) from the donor substrate UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA) on growing glycosaminoglycan chains. Increased expression of CSGALNACT2 has been observed in the arterial wall during the progression of atherosclerosis, contributing to the elongation of CS chains that may promote lipid retention and smooth muscle cell migration.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Methods Mol Biol; Bacterial Amyloids.; Authors - Margery L Evans