Placental lactogen Polyclonal antibody

Placental lactogen Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55236-1-AP

Synonyms

Choriomammotropin, CSA, CSH1, CSH2, CSMT, Lactogen, PL, Placental lactogen

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Validation Data Gallery

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  WB analysis of human placenta using 55236-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55236-1-AP (Placental lactogen antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 55236-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55236-1-AP (Placental lactogen antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 55236-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55236-1-AP (Placental lactogen antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 55236-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 55236-1-AP (Placental lactogen antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 55236-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 55236-1-AP (Placental lactogen antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55236-1-AP targets Placental lactogen in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: chorionic somatomammotropin hormone 1 (placental lactogen)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: NM_001317
• Gene Symbol: CSH1
• Gene ID (NCBI): 1442
• RRID: AB_10896996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSH1, also named as PL, CSH2 and Lactogen, belongs to the somatotropin/prolactin family. CSH1 produced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. It does not interact with GHR but only activates PRLR through zinc-induced dimerization(PubMed: 2744760). This antibody is specific to CSH1.