CSK Monoclonal antibody

CSK Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3B12C5

Cat no : 66115-1-Ig

Synonyms

Protein-tyrosine kinase CYL, EC:2.7.10.2, C-Src kinase, Csk A, C Src kinase

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Validation Data Gallery

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  WB analysis of Jurkat using 66115-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66115-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66115-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66115-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 66115-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CSK antibody (66115-1-Ig, Clone: 3B12C5 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66115-1-Ig targets CSK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CSK fusion protein Ag12103
• Full Name: c-src tyrosine kinase
• Calculated Molecular Weight: 450 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC106073
• Gene Symbol: CSK
• Gene ID (NCBI): 1445
• RRID: AB_2881514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).

Publications

Species	Application	Title
mouse	WB	Andrology; Trps1 targets Ccnd1 to regulate mouse Leydig cell proliferation.; Authors - Jiandong Sun