CSMD2 Polyclonal antibody

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  WB analysis using 31573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31573-1-AP (CSMD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 31573-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31573-1-AP (CSMD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 31573-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31573-1-AP (CSMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CSMD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31573-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31573-1-AP targets CSMD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36106

  Product name: Recombinant human CSMD2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 3246-3408 aa of NM_001281956

  Sequence: LEVRPSGRPINTAREPPLTQALIPGDVFAKNSLWKGAYEYQGKKQPAMLRVTGFQVANSKVNATMIDHSGVELHLAGTYKKEDFHLLLQVYQITGPVEIFMNKFKDDHWALDGHVSSESSGATFIYQGSVKGQGFGQFGFQRLDLRLLESDPESIGRHFASNS

• Full Name: CUB and Sushi multiple domains 2
• Calculated Molecular Weight: 380kDa
• Observed Molecular Weight: 380 kDa
• GenBank Accession Number: NM_001281956
• Gene Symbol: CSMD2
• Gene ID (NCBI): 114784
• RRID: AB_3670040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q7Z408
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CUB and sushi multiple domains 2 (CSMD2), located on the short arm of human chromosome 1 (1p35.1), encodes a single-pass transmembrane protein with a large extracellular domain consisting of CUB and Sushi domain repeats. CSMD2 is highly expressed in the developing and mature brain and has been linked to schizophrenia (PMID:31068362). The full-length molecular weight of CSMD2 protein is 380 kDa.