CSNK1A1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• CSNK1A1 Polyclonal antibody (55192-1-AP)

• Featured Product
• KD/KO Validated

CSNK1A1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 55192-1-AP Copied!

Publications (21)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

CK1, CK1α, CKI alpha, CKI-alpha, CSNK

• 

  WB analysis using 55192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 55192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 55192-1-AP

  WB result of CSNK1A1 antibody (55192-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSNK1A1 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 55192-1-AP

  IP result of anti-CSNK1A1 (IP:55192-1-AP, 4ug; Detection:55192-1-AP 1:4000) with HeLa cells lysate 960 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 55192-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CSNK1A1 antibody (55192-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 55192-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CSNK1A1 antibody (55192-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for CSNK1A1 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, Jurkat cells, MCF-7 cells, NIH/3T3 cells, U-87 MG cells, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55192-1-AP targets CSNK1A1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: casein kinase 1, alpha 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 30-35 kDa, 42-45 kDa
• GenBank Accession Number: NM_001892
• Gene Symbol: CSNK1A1
• Gene ID (NCBI): 1452
• RRID: AB_11183034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48729
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.

Publications

What published studies show

The anti-CSNK1A1/CK1α antibody (Cat# 55192-1-AP) has 21 citations published in journals including Nature Communications, Molecular Cell, Autophagy, Advanced Science, EMBO Reports, Journal of Cell Biology, and Cell Death & Disease. Associated research fields span cancer biology (hepatocellular, breast, colorectal), immunology and autoimmunity, circadian regulation, cardiovascular disease, metabolic disorders, reproductive biology, viral hepatitis, pulmonary fibrosis, stem cell biology, and mitochondrial function.

Automatically generated from the 21 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Autophagy; CSNK1A1/CK1α suppresses autoimmunity by restraining the CGAS-STING1 signaling; Authors - Mingyu Pan ; KD Validated
human	WB,IP,IF	Nat Commun; Phosphorylation-coupled autoregulation of TANGO1 and Sec16A maintains functional ER exit sites.; Authors - Miharu Maeda
		Mol Cell; BAG2 releases SAMD4B upon sensing of arginine deficiency to promote tumor cell survival; Authors - Meng-Ying Chen
human	WB	Adv Sci (Weinh); The PRMT6/PARP1/CRL4B Complex Regulates the Circadian Clock and Promotes Breast Tumorigenesis; Authors - Tianshu Yang
mouse	RIP,IHC	Cell Death Dis; CARMN loss promotes VSMC-derived foam cell formation and atherosclerosis through transcriptional downregulation of autophagy.; Authors - Tiegen Huang
human	WB,IHC	Pharmacol Res; Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex; Authors - Li Gu ; KD Validated