CSNK1G1 Polyclonal antibody

CSNK1G1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16384-1-AP

Synonyms

casein kinase 1, gamma 1, CKI gamma 1, CSNK1G1

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Validation Data Gallery

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  WB analysis using 16384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16384-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 16384-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16384-1-AP

  IP result of anti-CSNK1G1 (IP:16384-1-AP, 4ug; Detection:16384-1-AP 1:300) with Jurkat cells lysate 2800ug.

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  IHC staining of human liver using 16384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, COS-7 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

16384-1-AP targets CSNK1G1 in WB, IP, IHC, ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSNK1G1 fusion protein Ag9395
• Full Name: casein kinase 1, gamma 1
• Calculated Molecular Weight: 422 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC017236
• Gene Symbol: CSNK1G1
• Gene ID (NCBI): 53944
• RRID: AB_2087300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The casein kinase 1 (CK1) is a family of Ser/Thr protein kinases that functions as regulator in signal transduction of eukaryotic cells. CSNK1G1(Casein kinase I isoform gamma-1) plays an important role in activating the ATR/Chk1 pathway by phosphorylating Claspin, which was identified as a Chk1-activating protein. Recently, it has been found that CSNK1G1 can phosphorylate NF-kB subunit p65 at Ser536, which promotes its ubiquitin-mediated degredation by E3 ligases COMMD1 and CUL2.(PMID:24442433). This protein has 2 isoforms produced by alternative splicing with the molecular weight of 49 kDa and 45 kDa.

Publications

Species	Application	Title
human	WB,IP	J Immunol; Casein Kinase 1γ1 Inhibits the RIG-I/TLR Signaling Pathway through Phosphorylating p65 and Promoting Its Degradation.; Authors - Yetao Wang