CSNK2A1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83805-4-RR (same clone as 83805-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83805-4-RR (CSNK2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83805-4-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 83805-4-RR (same clone as 83805-4-PBS)

  WB result of CSNK2A1 antibody (83805-4-RR; 1:200000; room temperature for 1.5 hours) with wild-type and sg-CSNK2A1 transfected A549 cells. This data was developed using the same antibody clone with 83805-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83805-4-RR (same clone as 83805-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CSNK2A1 antibody (83805-4-RR, Clone: 240862A7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83805-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83805-4-RR (same clone as 83805-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CSNK2A1 antibody (83805-4-RR, Clone: 240862A7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83805-4-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 83805-4-RR (same clone as 83805-4-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CSNK2A1 Recombinant antibody (83805-4-RR, Clone:240862A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83805-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83805-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83805-4-RR against Human CSNK2A1 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

CSNK2A1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83805-4-PBS

Clone No.240862A7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240862A7, CK II alpha, CK2a, CK2A1, CK2α

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Product Information

83805-4-PBS targets CSNK2A1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1452

  Product name: Recombinant human CSNK2A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-391 aa of BC011668

  Sequence: MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ

• Full Name: casein kinase 2, alpha 1 polypeptide
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC011668
• Gene Symbol: CSNK2A1
• Gene ID (NCBI): 1457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P68400
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.