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- KD/KO Validated
CSPP1 Polyclonal antibody
CSPP1 Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, ELISA
Cat no : 11931-1-AP
Validation Data Gallery
|Positive WB detected in||Jurkat cells, HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 7 publications below|
|IHC||See 1 publications below|
|IF||See 8 publications below|
|IP||See 1 publications below|
11931-1-AP targets CSPP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CSPP1 fusion protein Ag2521|
|Full Name||centrosome and spindle pole associated protein 1|
|Calculated molecular weight||141 kDa|
|Observed molecular weight||141 kDa|
|GenBank accession number||BC029445|
|Gene ID (NCBI)||79848|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo
|Product Specific Protocols|
|WB protocol for CSPP1 antibody 11931-1-AP||Download protocol|
|IF protocol for CSPP1 antibody 11931-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Am J Hum Genet
Mutations in CSPP1 Cause Primary Cilia Abnormalities and Joubert Syndrome with or without Jeune Asphyxiating Thoracic Dystrophy.
Am J Hum Genet
Mutations in CSPP1 Lead to Classical Joubert Syndrome.
A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.
CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment.
The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.
Br J Cancer
Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.