CSPP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CSPP1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11931-1-AP

Synonyms

CSPP, CSPP1

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 11931-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Hela using 11931-1-AP

  Immunofluorescent analysis of Hela cells, using CSPP1 antibody 11931-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Product Information

11931-1-AP targets CSPP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSPP1 fusion protein Ag2521
• Full Name: centrosome and spindle pole associated protein 1
• Calculated molecular weight: 141 kDa
• Observed molecular weight: 141 kDa
• GenBank accession number: BC029445
• Gene symbol: CSPP1
• Gene ID (NCBI): 79848
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo

Publications

Species	Application	Title
human,mouse	IF	Cilia; Fixation methods can differentially affect ciliary protein immunolabeling.; Authors - Kiet Hua
human	WB,IF	Mol Biol Cell; The E3 ubiquitin ligase UBR5 regulates centriolar satellite stability and primary cilia.; Authors - Robert F Shearer
human	WB, IF	PLoS One; CSPP-L Associates with the Desmosome of Polarized Epithelial Cells and Is Required for Normal Spheroid Formation.; Authors - Johan Sternemalm
human,mouse	WB,IF,IP	Mol Biol Cell; CSPP is a ciliary protein interacting with Nephrocystin 8 and required for cilia formation.; Authors - Patzke Sebastian S
human	IHC,IF	Br J Cancer; Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.; Authors - J Sternemalm ; KD Validated
human	WB	Cell Rep; A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.; Authors - Kari-Anne M Frikstad ; KO Validated