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  • KD/KO Validated

CSPP1 Polyclonal antibody

CSPP1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11931-1-AP

Synonyms

CSPP, CSPP1



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11931-1-AP targets CSPP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSPP1 fusion protein Ag2521
Full Name centrosome and spindle pole associated protein 1
Calculated molecular weight 141 kDa
Observed molecular weight 141 kDa
GenBank accession numberBC029445
Gene symbol CSPP1
Gene ID (NCBI) 79848
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo

Protocols

Product Specific Protocols
WB protocol for CSPP1 antibody 11931-1-APDownload protocol
IF protocol for CSPP1 antibody 11931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Cilia

Fixation methods can differentially affect ciliary protein immunolabeling.

Authors - Kiet Hua
humanWB,IF

Mol Biol Cell

The E3 ubiquitin ligase UBR5 regulates centriolar satellite stability and primary cilia.

Authors - Robert F Shearer
humanWB, IF

PLoS One

CSPP-L Associates with the Desmosome of Polarized Epithelial Cells and Is Required for Normal Spheroid Formation.

Authors - Johan Sternemalm
human,mouseWB,IF,IP

Mol Biol Cell

CSPP is a ciliary protein interacting with Nephrocystin 8 and required for cilia formation.

Authors - Patzke Sebastian S
humanIHC,IF

Br J Cancer

Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.

Authors - J Sternemalm
  • KD Validated
humanWB

Cell Rep

A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.

Authors - Kari-Anne M Frikstad
  • KO Validated