- Featured Product
- KD/KO Validated
CSPP1 Polyclonal antibody
CSPP1 Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11931-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue |
Positive IF detected in | Hela cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 7 publications below |
IP | See 1 publications below |
Product Information
11931-1-AP targets CSPP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CSPP1 fusion protein Ag2521 |
Full Name | centrosome and spindle pole associated protein 1 |
Calculated molecular weight | 141 kDa |
Observed molecular weight | 141 kDa |
GenBank accession number | BC029445 |
Gene symbol | CSPP1 |
Gene ID (NCBI) | 79848 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CSPP1 antibody 11931-1-AP | Download protocol |
IF protocol for CSPP1 antibody 11931-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Biol Cell The E3 ubiquitin ligase UBR5 regulates centriolar satellite stability and primary cilia. | ||
PLoS One CSPP-L Associates with the Desmosome of Polarized Epithelial Cells and Is Required for Normal Spheroid Formation. | ||
Mol Biol Cell CSPP is a ciliary protein interacting with Nephrocystin 8 and required for cilia formation. | ||
Br J Cancer Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.
| ||
Cell Rep A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.
|