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  • KD/KO Validated

CSPP1 Polyclonal antibody

CSPP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11931-1-AP

Synonyms

CSPP, Centrosome and spindle pole-associated protein 1



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11931-1-AP targets CSPP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSPP1 fusion protein Ag2521
Full Name centrosome and spindle pole associated protein 1
Calculated Molecular Weight 141 kDa
Observed Molecular Weight 141 kDa
GenBank Accession NumberBC029445
Gene Symbol CSPP1
Gene ID (NCBI) 79848
RRIDAB_2087897
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo

Protocols

Product Specific Protocols
WB protocol for CSPP1 antibody 11931-1-APDownload protocol
IF protocol for CSPP1 antibody 11931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Am J Hum Genet

Mutations in CSPP1 Cause Primary Cilia Abnormalities and Joubert Syndrome with or without Jeune Asphyxiating Thoracic Dystrophy.

Authors - Karina Tuz
humanWB,IF

Am J Hum Genet

Mutations in CSPP1 Lead to Classical Joubert Syndrome.

Authors - Naiara Akizu
humanWB

Cell Rep

A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.

Authors - Kari-Anne M Frikstad
  • KO Validated
humanWB

EMBO Rep

CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment.

Authors - Cecilia Aquino Perez
  • KD Validated
humanIF

PLoS Biol

The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.

Authors - Umut Batman
humanIHC,IF

Br J Cancer

Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.

Authors - J Sternemalm
  • KD Validated