CSPP1 Polyclonal antibody

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  WB analysis of Jurkat using 11931-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11931-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11931-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11931-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 11931-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HEK-293 using 11931-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11931-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11931-1-AP (CSPP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 11931-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CSPP1 antibody (11931-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

CSPP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 11931-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CSPP, Centrosome and spindle pole-associated protein 1

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Product Information

11931-1-AP targets CSPP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSPP1 fusion protein Ag2521
• Full Name: centrosome and spindle pole associated protein 1
• Calculated Molecular Weight: 141 kDa
• Observed Molecular Weight: 141 kDa
• GenBank Accession Number: BC029445
• Gene Symbol: CSPP1
• Gene ID (NCBI): 79848
• RRID: AB_2087897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q1MSJ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo

Publications

Species	Application	Title
human	WB,IF	Am J Hum Genet; Mutations in CSPP1 Cause Primary Cilia Abnormalities and Joubert Syndrome with or without Jeune Asphyxiating Thoracic Dystrophy.; Authors - Karina Tuz
human	WB,IF	Am J Hum Genet; Mutations in CSPP1 Lead to Classical Joubert Syndrome.; Authors - Naiara Akizu
human	WB	Cell Rep; A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.; Authors - Kari-Anne M Frikstad ; KO Validated
human	WB	EMBO Rep; CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment.; Authors - Cecilia Aquino Perez ; KD Validated
human	IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
human	IHC,IF	Br J Cancer; Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.; Authors - J Sternemalm ; KD Validated