Tested Applications
| Positive WB detected in | human stomach tissue, HeLa cells |
| Positive IP detected in | mouse stomach tissue |
| Positive IHC detected in | human normal colon, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13432-1-AP targets CSRP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4236 Product name: Recombinant human CSRP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC032493 Sequence: MPNWGGGKKCGVCQKTVYFAEEVQCEGNSFHKSCFLCMVCKKNLDSTTVAVHGEEIYCKSCYGKKYGPKGYGYGQGAGTLSTDKGESLGIKHEEAPGHRPTTNPNASKFAQKIGGSERCPRCSQAVYAAEKVIGAGKSWHKACFRCAKCGKGLESTTLADKDGEIYCKGCYAKNFGPKGFGFGQGAGALVHSE Predict reactive species |
| Full Name | cysteine and glycine-rich protein 1 |
| Calculated Molecular Weight | 193 aa, 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC032493 |
| Gene Symbol | CSRP1 |
| Gene ID (NCBI) | 1465 |
| RRID | AB_2230180 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P21291 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSRP1 (Cysteine and glycine-rich protein 1), designated initially as CRP1, constitutes a cysteine-rich protein derived from placental sources and exhibits a response profile analogous to c-myc. CSRP1 has been recognized as a tumor suppressor in several types of cancers including colorectal cancer, and cholangiocarcinoma (PMID: 37113556). Abnormal expression of CSRP1 was reported within several malignancies such as prostate cancer and acute myeloid leukemia (PMID: 38813484, 40198006).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CSRP1 antibody 13432-1-AP | Download protocol |
| IP protocol for CSRP1 antibody 13432-1-AP | Download protocol |
| WB protocol for CSRP1 antibody 13432-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CSRP1 Polyclonal antibody (Cat# 13432-1-AP) has 3 citations published in Molecular Psychiatry, Molecular Medicine Reports, and FASEB Journal. The research fields span neurodevelopment (astrocyte morphogenesis and myelination), inflammatory/oxidative stress responses in chronic intermittent hypoxia, and tumor biology (neuroblastoma differentiation and apoptosis). Applications cited include Western blotting and immunofluorescence across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Mol Psychiatry PD-L1/PD-1 checkpoint pathway regulates astrocyte morphogenesis and myelination during brain development | ||
Mol Med Rep Inflammatory and oxidative stress‑associated factors in chronic intermittent hypoxia in Chinese patients, rats, lymphocytes and endotheliocytes. | ||
FASEB J The Involvement of CSRP1 in Neuroblastoma Differentiation and Apoptosis Impacting Tumor-Suppressive Therapeutic Responses |
