CSTF1 Polyclonal antibody

CSTF1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 15537-1-AP

Synonyms



Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, A549 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15537-1-AP targets CSTF1 in WB, IP, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSTF1 fusion protein Ag7874
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Calculated molecular weight 48 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC007425
Gene symbol CSTF1
Gene ID (NCBI) 1477
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 unit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD).

Protocols

Product Specific Protocols
WB protocol for CSTF1 antibody 15537-1-APDownload protocol
IF protocol for CSTF1 antibody 15537-1-APDownload protocol
IP protocol for CSTF1 antibody 15537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols