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  • KD/KO Validated

CTBP1 Polyclonal antibody

CTBP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10972-1-AP

Synonyms

EC:1.1.1.-, C-terminal-binding protein 1, CTBP, BARS



Tested Applications

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue, mouse thymus tissue, mouse brain tissue, HepG2 cells, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells, HeLa cekks
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10972-1-AP targets CTBP1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTBP1 fusion protein Ag1425
Full Name C-terminal binding protein 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC011655
Gene Symbol CTBP1
Gene ID (NCBI) 1487
RRIDAB_2086628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

Protocols

Product Specific Protocols
WB protocol for CTBP1 antibody 10972-1-APDownload protocol
IHC protocol for CTBP1 antibody 10972-1-APDownload protocol
IF protocol for CTBP1 antibody 10972-1-APDownload protocol
IP protocol for CTBP1 antibody 10972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression.

Authors - Yongqing Liu
humanWB

Nat Commun

ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin.

Authors - Lifang Li
  • KD Validated
humanWB,IF

Oncogene

RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.

Authors - Yang Yu
humanWB

Mol Oncol

Role of hepatoma-derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma.

Authors - Xuejie Min
humanWB

Mol Carcinog

Role of miR-214 in regulation of β-catenin and the malignant phenotype of melanoma.

Authors - Kirthana Prabhakar
  • KD Validated
mouseIF

Curr Mol Med

Toosendanin Restrains Idiopathic Pulmonary Fibrosis by Inhibiting ZEB1/CTBP1 Interaction

Authors - Xingbin Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (02-18-2020)

This antibody worked perfectly. I had a specific band at ~48kD, which is at the predicted size of Ctbp1. Highly recommended for detecting Ctbp1 by western blots!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart