Tested Applications
| Positive WB detected in | HEK-293 cells, mouse skeletal muscle tissue, mouse thymus tissue, mouse brain tissue, HepG2 cells, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells, HeLa cekks |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human prostate cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10972-1-AP targets CTBP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1425 Product name: Recombinant human CTBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-440 aa of BC011655 Sequence: MGSSHLLNKGLPLGVRPPIMNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRATWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGVERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYRYPPGVVGVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHASDQL Predict reactive species |
| Full Name | C-terminal binding protein 1 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC011655 |
| Gene Symbol | CTBP1 |
| Gene ID (NCBI) | 1487 |
| RRID | AB_2086628 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13363 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CTBP1 antibody 10972-1-AP | Download protocol |
| IF protocol for CTBP1 antibody 10972-1-AP | Download protocol |
| IHC protocol for CTBP1 antibody 10972-1-AP | Download protocol |
| IP protocol for CTBP1 antibody 10972-1-AP | Download protocol |
| WB protocol for CTBP1 antibody 10972-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CTBP1 antibody (Cat# 10972-1-AP) has 12 citations across journals including Nature Communications, Oncogene, Clinical and Translational Medicine, Molecular Oncology, Frontiers in Pharmacology, Frontiers in Physiology, Molecular Carcinogenesis, Cell Biochemistry and Biophysics, Current Molecular Medicine, Biochemical and Biophysical Research Communications, and Molecular and Cellular Biochemistry. Research fields span epilepsy, gastric cancer, ferroptosis in lung cancer, adipocyte browning/obesity, hepatocellular carcinoma, melanoma, Wnt/β-catenin signaling, pulmonary fibrosis, breast carcinoma, and diabetic cardiomyopathy.
| Species | Application | Title |
|---|---|---|
Nat Commun CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression. | ||
Nat Commun ZNF516 suppresses EGFR by targeting the CtBP/LSD1/CoREST complex to chromatin.
| ||
Oncogene RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer. | ||
Clin Transl Med Targeting the CTBP1-CETP axis overcomes ferroptosis resistance in non-small cell lung cancer by altering lipid accumulation.
| ||
Front Pharmacol Ling-gui-zhu-gan promotes adipocytes browning via targeting the miR-27b/PRDM16 pathway in 3T3-L1 cells | ||
Mol Oncol Role of hepatoma-derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma. |
Reviews
What customers say
The single review for this antibody is a 5-star rating. The user performed a Western Blot on mouse heart tissue at a 1:1000 dilution and reported a specific band at approximately 48 kDa, matching the predicted size of Ctbp1. They described the antibody as working perfectly and highly recommended it for detecting Ctbp1 by Western blot.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tongbin (Verified Customer) (02-18-2020) | This antibody worked perfectly. I had a specific band at ~48kD, which is at the predicted size of Ctbp1. Highly recommended for detecting Ctbp1 by western blots!
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