Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse skeletal muscle tissue, mouse thymus tissue, mouse brain tissue, HepG2 cells, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells, HeLa cekks|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10972-1-AP targets CTBP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CTBP1 fusion protein Ag1425|
|Full Name||C-terminal binding protein 1|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC011655|
|Gene ID (NCBI)||1487|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.
|Product Specific Protocols|
|WB protocol for CTBP1 antibody 10972-1-AP||Download protocol|
|IHC protocol for CTBP1 antibody 10972-1-AP||Download protocol|
|IF protocol for CTBP1 antibody 10972-1-AP||Download protocol|
|IP protocol for CTBP1 antibody 10972-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tongbin (Verified Customer) (02-18-2020)
This antibody worked perfectly. I had a specific band at ~48kD, which is at the predicted size of Ctbp1. Highly recommended for detecting Ctbp1 by western blots!