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CTLA-4 Polyclonal antibody

CTLA-4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30648-1-AP

Synonyms

Cd152, Ctla 4, Ctla4, Ly 56

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTLA-4 Polyclonal antibody (30648-1-AP)

    WB analysis using 30648-1-AP

    Untreated or Con A-treated (3 ug/ml, 72h) mouse splenocytes were subjected to SDS PAGE followed by western blot with 30648-1-AP (CTLA-4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inCon A treated mouse splenocytes

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30648-1-AP targets CTLA-4 in WB, ELISA applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein
Full Name cytotoxic T-lymphocyte-associated protein 4
Calculated Molecular Weight25 kDa
Observed Molecular Weight28-40 kDa
GenBank Accession NumberNM_009843.4
Gene Symbol Ctla4
Gene ID (NCBI) 12477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).

Protocols

Product Specific Protocols
WB protocol for CTLA-4 antibody 30648-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols