Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of mouse splenocytes using 98147-1-RR (same clone as 98147-1-PBS)

  1x10^6 untreated or Con A treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were not fixed. This data was developed using the same antibody clone with 98147-1-PBS in a different storage buffer formulation.

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  FC experiment of mouse splenocytes using 98147-1-RR (same clone as 98147-1-PBS)

  1x10^6 Con A treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8)(red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98147-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98147-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98147-1-RR against Mouse CTLA-4/CD152 were performed. The affinity constant is 0.580 nM.

CTLA-4/CD152 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98147-1-PBS

Clone No.241100B8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Ctla4, Ly 56, Ctla 4, Cd152, 241100B8

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Product Information

98147-1-PBS targets CTLA-4/CD152 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: cytotoxic T-lymphocyte-associated protein 4
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: NM_009843.4
• Gene Symbol: CTLA-4
• Gene ID (NCBI): 12477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P09793
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).