Alpha E-Catenin Monoclonal antibody

Alpha E-Catenin Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B7B8

Cat no : 66221-1-Ig

Synonyms

Alpha E catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1



Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IHC detected inhuman stomach tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66221-1-Ig targets Alpha E-Catenin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alpha E-Catenin fusion protein Ag23603
Full Name catenin (cadherin-associated protein), alpha 1, 102kDa
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC031262
Gene symbol CTNNA1
Gene ID (NCBI) 1495
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

Protocols

Product Specific Protocols
WB protocol for Alpha E-Catenin antibody 66221-1-IgDownload protocol
IHC protocol for Alpha E-Catenin antibody 66221-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Int J Mol Sci

Histological and Immunohistochemical Studies to Determine the Mechanism of Cleft Palate Induction after Palatal Fusion in Mice Exposed to TCDD.

Authors - Chisato Sakuma
humanWB

Oncotarget

YAP1 enhances cell proliferation, migration, and invasion of gastric cancer in vitro and in vivo.

Authors - Dan Sun