|Positive WB detected in||NIH/3T3 cells|
|Positive IHC detected in||human stomach tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:1000|
|Sample-dependent, check data in validation data gallery|
66221-1-Ig targets Alpha E-Catenin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Alpha E-Catenin fusion protein Ag23603|
|Full Name||catenin (cadherin-associated protein), alpha 1, 102kDa|
|Calculated molecular weight||906 aa, 100 kDa|
|Observed molecular weight||95-100 kDa|
|GenBank accession number||BC031262|
|Gene ID (NCBI)||1495|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.
YAP1 enhances cell proliferation, migration, and invasion of gastric cancer in vitro and in vivo.
Int J Mol Sci
Histological and Immunohistochemical Studies to Determine the Mechanism of Cleft Palate Induction after Palatal Fusion in Mice Exposed to TCDD.
Mol Med Rep
Wild-type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β-catenin pathway