|Positive WB detected in||NIH/3T3 cells|
|Positive IHC detected in||human stomach tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:1000|
|Sample-dependent, check data in validation data gallery|
66221-1-Ig targets Alpha E-Catenin in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Alpha E-Catenin fusion protein Ag23603|
|Full Name||catenin (cadherin-associated protein), alpha 1, 102kDa|
|Calculated molecular weight||906 aa, 100 kDa|
|Observed molecular weight||95-100 kDa|
|GenBank accession number||BC031262|
|Gene ID (NCBI)||1495|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.
Int J Mol Sci
Histological and Immunohistochemical Studies to Determine the Mechanism of Cleft Palate Induction after Palatal Fusion in Mice Exposed to TCDD.
YAP1 enhances cell proliferation, migration, and invasion of gastric cancer in vitro and in vivo.
Mol Med Rep
Wild-type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β-catenin pathway