CTNNA3 Polyclonal antibody

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• CTNNA3 Polyclonal antibody (13974-1-AP)

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• KD/KO Validated

CTNNA3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13974-1-AP Copied!

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Alpha T catenin, Alpha T-catenin, Cadherin-associated protein, Catenin alpha 3, Catenin alpha-3

• 

  WB analysis of HEK-293 using 13974-1-AP

  WB result of CTNNA3 antibody (13974-1-AP, 1:500) with si-Control and si-CTNNA3 transfected HEK293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 13974-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13974-1-AP (CTNNA3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse testis using 13974-1-AP

  IP result of anti-CTNNA3 (IP:13974-1-AP, 5ug; Detection:13974-1-AP 1:1500) with mouse testis tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 13974-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13974-1-AP (CTNNA3 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 13974-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13974-1-AP (CTNNA3 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 13974-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13974-1-AP (CTNNA3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 13974-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13974-1-AP (CTNNA3 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 13974-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13974-1-AP (CTNNA3 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 13974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13974-1-AP (CTNNA3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse testis tissue, HEK-293
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human liver cancer tissue, human heart tissue, human placenta tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13974-1-AP targets CTNNA3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5008

  Product name: Recombinant human CTNNA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-376 aa of BC030646

  Sequence: MSAETPITLNIDPQDLQVQTFTVEKLLEPLIIQVTTLVNCPQNPSSRKKGRSKRASVLLASVEEATWNLLDKGEKIAQEATVLKDELTASLEKVRKESEALKVSAERFTDDPCFLPKREAVVQAARALLAAVTRLLILADMIDVMCLLQHVSAFQRTFESLKNVANKSDLQKTYQKLGKELENLDYLAFKRQQDLKSPNQRDEIAGARASLKENSPLLHSICSACLEHSDVASLKASKDTVCEEIQNALNVISNASQGIQNMTTPPEPQAATLGSALDELENLIVLNPLTVTEEEIRPSLEKRLEAIISGAALLADSSCTRDLHRERIIAECNAIRQALQDLLSEYMNNLCNQKSDLGLTGVKIKLLEECIIFWSL

• Full Name: catenin (cadherin-associated protein), alpha 3
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC030646
• Gene Symbol: CTNNA3
• Gene ID (NCBI): 29119
• RRID: AB_2088078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UI47
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. The alpha catenin family of proteins consists of three members, alpha-E-catenin (CTNNA1), alpha-N-catenin (CTNNA2), and alpha-T-catenin (CTNNA3)(PMID: 21598020). CTNNA3 is a cell adhesion molecule. In intercalated discs of the heart, CTNNA3 is a component of a unique hybrid adhering junction, or area composita, that contains proteins associated with both desmosomes and adherens junctions (PMID: 22421363). Mutations in CTNNA3 are associated with arrhythmogenic right ventricular dysplasia, familial 13(PMID: 23136403).

Publications

What published studies show

Anti-α-Catenin antibody (Cat# 13974-1-AP) has 14 total citations appearing in journals including Nature, Cell Stem Cell, Nature Communications, Circulation Research, Development, EMBO Reports, iScience, J Cell Sci, and eLife. Associated research fields span cardiac biology (Yap signaling, cardiomyocyte proliferation, myocardial regeneration), cytoskeletal dynamics, cell adhesion, cancer (laryngeal carcinoma, glioblastoma), muscle stem cells, and HIV–host protein interactions.

Automatically generated from the 14 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; Global landscape of HIV-human protein complexes.; Authors - Stefanie Jäger
mouse	IF	Cell Stem Cell; An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling.; Authors - Allison P Kann
human	WB	Nat Commun; Cell-cell adhesion genes CTNNA2 and CTNNA3 are tumour suppressors frequently mutated in laryngeal carcinomas.; Authors - Fanjul-Fernández Miriam M ; KD Validated
mouse	IF	Circ Res; Alpha-Catenins Control Cardiomyocyte Proliferation by Regulating Yap Activity.; Authors - Jifen Li
mouse	WB	Acta Pharm Sin B; Transient induction of actin cytoskeletal remodeling associated with dedifferentiation, proliferation, and redifferentiation stimulates cardiac regeneration; Authors - Wenbin Fu
human	WB	EMBO Rep; Praja2 controls P-body assembly and translation in glioblastoma by non-proteolytic ubiquitylation of DDX6; Authors - Emanuela Senatore