CTNNBL1 Polyclonal antibody

CTNNBL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13665-1-AP

Synonyms

Beta catenin like protein 1, C20orf33, catenin, beta like 1, CTNNBL1, NAP, Nuclear associated protein, NYD SP19, P14L, PP8304

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Validation Data Gallery

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  WB analysis of Jurkat using 13665-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13665-1-AP (CTNNBL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13665-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13665-1-AP (CTNNBL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13665-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13665-1-AP (CTNNBL1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 13665-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13665-1-AP (CTNNBL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 13665-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13665-1-AP (CTNNBL1 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse spleen tissue, mouse thymus tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

13665-1-AP targets CTNNBL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTNNBL1 fusion protein Ag4578
• Full Name: catenin, beta like 1
• Calculated Molecular Weight: 563 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC036739
• Gene Symbol: CTNNBL1
• Gene ID (NCBI): 56259
• RRID: AB_2086245
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTNNBL1, also named as C20orf33, belongs to the armadillo-containing protein family (PMID: 32484799). CTNNBL1 is a widely expressed nucleoprotein that associates with the pre-mRNA processing factor 19 (Prp19) complex of the spliceosome, interacting with its CDC5L component. CTNNBL1 can maintain the content and integrity of the Prp19 spliceosome complex by regulating the interaction between CWC15 and CDC5L (PMID: 28501461). CTNNBL1 has 4 isoforms with the molecular weights of 36, 43, 62 and 65 kDa, respectively. CTNNBL1 may play an important role in colorectal cancer and regulating AID function (PMID: 28501461, 32484799).

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Role of Glycated High Mobility Group Box-1 in Gastric Cancer.; Authors - Shingo Kishi
human	WB	J Transl Med; eIF6 promotes the malignant progression of human hepatocellular carcinoma via the mTOR signaling pathway.; Authors - Liping Sun