CTNS Polyclonal antibody

CTNS Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13085-1-AP

Synonyms

CTNS, CTNS LSB, Cystinosin, cystinosis, nephropathic, PQLC4

---

Validation Data Gallery

• 

  WB analysis using 13085-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13085-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13085-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13085-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13085-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 13085-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CTNS antibody (13085-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 13085-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 13085-1-AP (CTNS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

13085-1-AP targets CTNS in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTNS fusion protein Ag3904
• Full Name: cystinosis, nephropathic
• Calculated Molecular Weight: 400aa,45 kDa; 367aa,42 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC032850
• Gene Symbol: CTNS
• Gene ID (NCBI): 1497
• RRID: AB_2230084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CTNS or cystinosin is a lysosomal membrane protein which comprises with seven transmembrane domains, a 128 amino acid N-terminal region bearing seven N-glycosylation sites and a cytosolic C-terminal GYDQL sorting motif. It functions as a H+-driven cystine transporter responsible for cystine export from lysosomes. The mutation of CTNS gene cause an inherited disorder, cystinosis, characterized by defective lysosomal efflux of cystine. This antibody was raised against the N-terminal region of human cystinosin. Two isoforms of CTNS exist due to alternative splicing events and both of them can be detected through this antibody.

Publications

Species	Application	Title
	WB	Biochem Biophys Res Commun; Reduced phosphate transport in the renal proximal tubule cells in cystinosis is due to decreased expression of transporters rather than an energy defect.; Authors - Mary L Taub
human	WB	Biochem Biophys Res Commun; Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.; Authors - Jong-Jer Lee
mouse	IF	J Immunol Methods; Characterization of a rat monoclonal antibody raised against ferroptotic cells.; Authors - Sho Kobayashi