Filter: AllWBSandwich ELISAIHCIF-PIF/ICC

WB analysis using 67734-1-Ig (same clone as 67734-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67734-1-Ig (CTPS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.
×
Various lysates were subjected to SDS PAGE followed by western blot with 67734-1-Ig (CTPS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.
WB analysis of U2OS using 67734-1-Ig (same clone as 67734-1-PBS)
U2OS cells were subjected to SDS PAGE followed by western blot with 67734-1-Ig (CTPS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.
Sandwich ELISA standard curve of MP50097-1
Sandwich ELISA standard curve of MP50097-1, CTPS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67734-1-PBS. Detection antibody: 67734-2-PBS. Standard:Ag30258. Range: 0.195-50 ng/mL.
IHC staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67734-1-Ig (CTPS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67734-1-Ig (CTPS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.
IF Staining of human prostate cancer using 67734-1-Ig (same clone as 67734-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.
IF Staining of PC-12 using 67734-1-Ig (same clone as 67734-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using CTPS2 antibody (67734-1-Ig, Clone: 1A3B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67734-1-PBS in a different storage buffer formulation.

CTPS2 Monoclonal antibody, PBS Only (Capture)

CTPS2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA

Cat No. 67734-1-PBS

Clone No.1A3B8

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA

Synonyms

1A3B8, CTP synthase 2, CTP synthase II, CTP synthetase 2, EC:6.3.4.2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

67734-1-PBS targets CTPS2 as part of a matched antibody pair:

MP50097-1: 67734-1-PBS capture and 67734-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag30258 Product name: Recombinant human CTPS2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 30-373 aa of BC034986 Sequence: CGLRVTAIKIDPYINIDAGTFSPYEHGEVFVLNDGGEVDLDLGNYERFLDINLYKDNNITTGKIYQHVINKERRGDYLGKTVQVVPHITDAVQEWVMNQAKVPVDGNKEEPQICVIELGGTIGDIEGMPFVEAFRQFQFKAKRENFCNIHVSLVPQLSATGEQKTKPTQNSVRALRGLGLSPDLIVCRSSTPIEMAVKEKISMFCHVNPEQVICIHDVSSTYRVPVLLEEQSIVKYFKERLHLPIGDSASNLLFKWRNMADRYERLQKICSIALVGKYTKLRDCYASVFKALEHSALAINHKLNLMYIDSIDLEKITETEDPVKFHEAWQKLCKADGILVPGGF Predict reactive species
Full Name	CTP synthase II
Calculated Molecular Weight	586 aa, 66 kDa
Observed Molecular Weight	66 kDa
GenBank Accession Number	BC034986
Gene Symbol	CTPS2
Gene ID (NCBI)	56474
RRID	AB_2882918
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9NRF8
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CTPS2 (CTP synthase 2) is also named as UTP--ammonia ligase 2 and belongs to the CTP synthase family.It catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen and constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides. This antibody specifically detects only CTPS2 and not also CTPS1.