Chymotrypsin C Polyclonal antibody

• Home
• Products
• Primary Antibodies
• Chymotrypsin C Polyclonal antibody (16416-1-AP)

• 

  WB analysis using 16416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16416-1-AP (Chymotrypsin C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16416-1-AP (Chymotrypsin C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse pancreas using 16416-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 16416-1-AP (Chymotrypsin C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 16416-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 16416-1-AP (Chymotrypsin C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 16416-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 16416-1-AP (Chymotrypsin C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Chymotrypsin C Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16416-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CTRC, Caldecrin, Chymotrypsin-C, CLCR, EC:3.4.21.2

---

Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas tissue
• Positive IHC detected in: mouse pancreas tissue, rat pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16416-1-AP targets Chymotrypsin C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9572

  Product name: Recombinant human CTRC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-268 aa of BC015118

  Sequence: MLGITVLAALLACASSCGVPSFPPNLSARVVGGEDARPHSWPWQISLQYLKNDTWRHTCGGTLIASNFVLTAAHCISNTRTYRVAVGKNNLEVEDEEGSLFVGVDTIHVHKRWNALLLRNDIALIKLAEHVELSDTIQVACLPEKDSLLPKDYPCYVTGWGRLWTNGPIADKLQQGLQPVVDHATCSRIDWWGFRVKKTMVCAGGDGVISACNGDSGGPLNCQLENGSWEVFGIVSFGSRRGCNTRKKPVVYTRVSAYIDWINEKMQL

• Full Name: chymotrypsin C (caldecrin)
• Calculated Molecular Weight: 268 aa, 29 kDa
• Observed Molecular Weight: 30 kDa, 35 kDa
• GenBank Accession Number: BC015118
• Gene Symbol: CTRC
• Gene ID (NCBI): 11330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99895
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chymotrypsin C (chymotrypsin C) is a serine protease of digestive tract encoded by human CTRC gene. Secreted by pancreatic acinar cells, it is not only involved in the protein degradation of food, but also the "protector" of the pancreas, which can prevent the premature activation of trypsin in the pancreas by degrading trypsinogen. If the mutation of CTRC gene leads to the loss of protein's function, it will destroy the original protective degradation process of trypsinogen, and lead to the proliferation of abnormally activated trypsin in the pancreas, thus significantly increasing the risk of hereditary or idiopathic chronic pancreatitis (PMID: 35594281). The molecular weight of the protein is 30 kDa(PMID: 21828354), and there is N- glycosylation modification, and the modified molecular weight is 35kDa. The same protein may present different glycosylation forms in two species (PMID: 34118474).