Cathepsin D Polyclonal antibody

Cathepsin D Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55021-1-AP

Synonyms

Cathepsin D, CLN10, CPSD, CTSD



Tested Applications

Positive WB detected inA431 cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55021-1-AP targets Cathepsin D in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cathepsin D
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberNM_001909
Gene Symbol CTSD
Gene ID (NCBI) 1509
RRIDAB_10863128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSD (Cathepsin D) is also named as CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. (PMID: 27114232, PMID: 30717773, PMID: 30051532)

Protocols

Product Specific Protocols
WB protocol for Cathepsin D antibody 55021-1-APDownload protocol
IHC protocol for Cathepsin D antibody 55021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Acta Neuropathol Commun

Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease

Authors - Suman Rimal
humanWB

Toxicology

1,3-dichloro-2-propanol induced lipid accumulation by blocking autophagy flux in HepG2 cells.

Authors - Jing Lu
humanIF

Pathogens

Sputum from People with Cystic Fibrosis Reduces the Killing of Methicillin-Resistant Staphylococcus aureus by Neutrophils and Diminishes Phagosomal Production of Reactive Oxygen Species

Authors - Kayla M Fantone
humanWB

Cell Death Discov

A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein.

Authors - Pengyun Li
humanWB

Chem Biol Interact

In addition to its endosomal escape effect, platycodin D also synergizes with ribosomal inactivation protein to induce apoptosis in hepatoma cells through AKT and MAPK signaling pathways.

Authors - Jin Lei
humanWB

Biochem Biophys Res Commun

Bifendate inhibits autophagy at multiple steps and attenuates oleic acid-induced lipid accumulation

Authors - Weigang Yuan