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Cathepsin L Polyclonal antibody

Cathepsin L Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10938-1-AP

Synonyms

Cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, Major excreted protein, MEP



Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10938-1-AP targets Cathepsin L in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cathepsin L fusion protein Ag1373
Full Name cathepsin L1
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 36-39 kDa, 29 kDa
GenBank Accession NumberBC012612
Gene Symbol CTSL1
Gene ID (NCBI) 1514
RRIDAB_2087834
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

Protocols

Product Specific Protocols
WB protocol for Cathepsin L antibody 10938-1-APDownload protocol
IHC protocol for Cathepsin L antibody 10938-1-APDownload protocol
IF protocol for Cathepsin L antibody 10938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Emerg Microbes Infect

GILT restricts the cellular entry mediated by the envelope glycoproteins of SARS-CoV, Ebola virus and Lassa fever virus.

Authors - Danying Chen
humanIF

Front Microbiol

Cronobacter sakazakii ATCC 29544 Translocated Human Brain Microvascular Endothelial Cells via Endocytosis, Apoptosis Induction, and Disruption of Tight Junction.

Authors - Tong Jin
humanWB

J Cancer Res Clin Oncol

Expressions and significances of CTSL, the target of COVID-19 on GBM.

Authors - Qiang Dong
humanWB

Cancer Cell Int

Differential secretome of pancreatic cancer cells in serum-containing conditioned medium reveals CCT8 as a new biomarker of pancreatic cancer invasion and metastasis.

Authors - Peng Liu
humanIHC

Exp Eye Res

A proteomic approach towards understanding the pathogenesis of Mooren's ulcer.

Authors - Hao Chi
humanIHC

BMC Infect Dis

COVID-19: a fatal case of acute liver failure associated with SARS-CoV-2 infection in pre-existing liver cirrhosis.

Authors - Jana Ihlow