CTSZ Polyclonal antibody

CTSZ Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16578-1-AP

Synonyms

Cathepsin P, Cathepsin X, cathepsin Z, CTSX, CTSZ

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Validation Data Gallery

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  WB analysis using 16578-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16578-1-AP (CTSZ antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 16578-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16578-1-AP (CTSZ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16578-1-AP

  IP result of anti-CTSZ (IP:16578-1-AP, 4ug; Detection:16578-1-AP 1:300) with HeLa cells lysate 2500ug.

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  IHC staining of human pancreas using 16578-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16578-1-AP (CTSZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 16578-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16578-1-AP (CTSZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

16578-1-AP targets CTSZ in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTSZ fusion protein Ag9865
• Full Name: cathepsin Z
• Calculated Molecular Weight: 303 aa, 34 kDa
• Observed Molecular Weight: 34-37 kDa
• GenBank Accession Number: BC042168
• Gene Symbol: CTSZ
• Gene ID (NCBI): 1522
• RRID: AB_2878282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
mouse	WB,IF	Alzheimers Dement; Urolithin A improves Alzheimer's disease cognition and restores mitophagy and lysosomal functions; Authors - Yujun Hou