CTTNBP2 Polyclonal antibody

CTTNBP2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17893-1-AP

Synonyms

C7orf8, cortactin binding protein 2, CORTBP2, CTTNBP2, KIAA1758, Orf4

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Validation Data Gallery

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  WB analysis of mouse brain using 17893-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17893-1-AP (CTTNBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17893-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17893-1-AP (CTTNBP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17893-1-AP

  IP result of anti-CTTNBP2 (IP:17893-1-AP, 5ug; Detection:17893-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human testis using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse kidney tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human testis tissue, human brain tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

17893-1-AP targets CTTNBP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTTNBP2 fusion protein Ag12219
• Full Name: cortactin binding protein 2
• Calculated Molecular Weight: 1663 aa, 181 kDa
• Observed Molecular Weight: 181 kDa
• GenBank Accession Number: BC106000
• Gene Symbol: CTTNBP2
• Gene ID (NCBI): 83992
• RRID: AB_2230211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; Social deficits in Shank3-deficient mouse models of autism are rescued by histone deacetylase (HDAC) inhibition.; Authors - Luye Qin
human	IHC	Mod Pathol; Expression profiling of small intestinal neuroendocrine tumors identifies subgroups with clinical relevance, prognostic markers and therapeutic targets.; Authors - Ellinor Andersson
human	IF	Sci Adv; Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression; Authors - Hao Zhang