CTTNBP2NL Polyclonal antibody

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• CTTNBP2NL Polyclonal antibody (25523-1-AP)

CTTNBP2NL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 25523-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA
For FC, select conjugated version.

Synonyms

CTTNBP2 N terminal like, CTTNBP2 N-terminal-like protein, KIAA1433

• 

  WB analysis using 25523-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25523-1-AP (CTTNBP2NL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MDA-MB-453s using 25523-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25523-1-AP (CTTNBP2NL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skin using 25523-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 25523-1-AP (CTTNBP2NL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of MCF-7 using 25523-1-AP

  IP result of anti-CTTNBP2NL (IP:25523-1-AP, 4ug; Detection:25523-1-AP 1:600) with MCF-7 cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 25523-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25523-1-AP (CTTNBP2NL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 25523-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CTTNBP2NL antibody (25523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 25523-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CTTNBP2NL antibody (25523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, MDA-MB-453s cells, mouse skin tissue, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25523-1-AP targets CTTNBP2NL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22137

  Product name: Recombinant human CTTNBP2NL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 271-479 aa of BC016029

  Sequence: KKIVKDLEASHQHSSPNEQLKKPVTVSKGTATEPLMLMSVFCQTESFPAERTHGSNIAKMTNTGLPGPATPAYSYAKTNGHCDPEIQTTRELTAGNNVENQVPPREKSVALAQEKPVENGGCPVGIETPVPMPSPLSSSGSSLSPSSTASSSLTSSPCSSPVLTKRLLGSSASSPGYQSSYQVGINQRFHAARHKFQSQADQDQQASGL

• Full Name: CTTNBP2 N-terminal like
• Calculated Molecular Weight: 639 aa, 70 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC016029
• Gene Symbol: CTTNBP2NL
• Gene ID (NCBI): 55917
• RRID: AB_2880117
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2B4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The CTTNBP2NL Polyclonal antibody (Cat# 25523-1-AP) has 3 citations published in Frontiers in Pharmacology, PLoS Pathogens, and Cytojournal. The research fields span oncology (clear cell renal cell carcinoma and thyroid cancer), virology (enterovirus infection proteolytic landscape), and apoptosis regulation. All cited studies used human samples with WB and/or IF applications.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Front Pharmacol; Tat-HSPE1 suppresses clear cell renal cell carcinoma growth through lysosome-dependent cell death.; Authors - Lin Zhang ; KD Validated
human	WB	PLoS Pathog; Defining the proteolytic landscape during enterovirus infection.; Authors - Mohsan Saeed
human	WB	Cytojournal; Transcriptional enhanced associate domain factor 1 regulates cortactin-binding protein 2 N-terminal-like to control cell apoptosis in thyroid cancer.; Authors - Qian Ma ; KD Validated