CUGBP2 Polyclonal antibody

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  WB analysis using 12921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 12921-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12921-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 12921-1-AP

  IP result of anti-CUGBP2 (IP:12921-1-AP, 3ug; Detection:12921-1-AP 1:300) with mouse skeletal muscle tissue lysate 7500ug.

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  IHC staining of human breast cancer using 12921-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12921-1-AP (CUGBP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12921-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12921-1-AP (CUGBP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12921-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12921-1-AP (CUGBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CUGBP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12921-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CELF-2, CELF2, CELF 2, Bruno-like protein 3, BRUNOL3

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Tested Applications

• Positive WB detected in: HeLa cells, mouse thymus tissue, K-562 cells, MCF-7 cells
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12921-1-AP targets CUGBP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3594

  Product name: Recombinant human CUGBP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 175-521 aa of BC036391

  Sequence: STRAMAQNAIKAMHQSQTMEGCSSPIVVKFADTQKDKEQRRLQQQLAQQMQQLNTATWGNLTGLGGLTPQYLALLQQATSSSNLGAFSGIQQMAGMNALQLQNLATLAAAAAAAQTSATSTNANPLSTTSSALGALTSPVAASTPNSTAGAAMNSLTSLGTLQGLAGATVGLNNINALAGTINTPRSKRLLLPKDNNGMAALNGGLGATGLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLLQQQSAAGSQKEGPEGANLFIYHLPQEFGDQDILQMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQAMNGFQIGMKRLKVQLKRSKNDSKPY

• Full Name: CUG triplet repeat, RNA binding protein 2
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC036391
• Gene Symbol: CUGBP2
• Gene ID (NCBI): 10659
• RRID: AB_2230041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95319
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CELF (CUGBP and ETR-3 Like Factor)/Bruno-like protein family plays important roles in the regulation of alternative splicing and translation. CUGBP2 is a member of this family of proteins that possesses several alternatively spliced exons. CUGBP2 induces smooth muscle-specific exon inclusion via binding to uridine purine repeat elements (URE) in ACTN1. Although CUGBP2 is known as a splicing activator for the N-methyl-D-aspartate receptor 1 (NMDA R1) exon 21, CUGBP2 represses NMDA R1 exon 5 inclusion. In addition, CUGBP2 was reported to repress the inclusion of the Ir exon 11.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncotarget; Inhibition of COX2 enhances the chemosensitivity of dichloroacetate in cervical cancer cells.; Authors - Bo Li
mouse	IHC	Brain Res; Transcriptional induction and translational inhibition of Arc and Cugbp2 in mice hippocampus after transient global ischemia under normothermic condition.; Authors - Otsuka Noboru N
human	WB	Exp Biol Med (Maywood); Hypoxic colorectal cancer-secreted exosomes deliver miR-210-3p to normoxic tumor cells to elicit a protumoral effect.; Authors - Liuqing Ge
human	WB,IF	Pharmacol Res; Exosomal miR-625-3p secreted by cancer-associated fibroblasts in colorectal cancer promotes EMT and chemotherapeutic resistance by blocking the CELF2/WWOX pathway; Authors - Ying Zhang
human	IF	bioRxiv; LINC01432 binds to CELF2 in newly diagnosed multiple myeloma promoting short progression-free survival to standard therapy; Authors - Richa Mishra