CUL3 Recombinant antibody, PBS Only

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  WB analysis using 83619-5-RR (same clone as 83619-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83619-5-RR (CUL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83619-5-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 83619-5-RR (same clone as 83619-5-PBS)

  IP result of anti-CUL3 (IP:83619-5-RR, 4ug; Detection:83619-5-RR 1:4000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 83619-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83619-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83619-5-RR against Human CUL3 were performed. The affinity constant is below 1 pM.

CUL3 Uni-rAb^TM Recombinant Antibody for WB, IP, ELISA

Cat No. 83619-5-PBS

Clone No.240534C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

240534C7, CUL-3, Cullin-3, KIAA0617

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Product Information

83619-5-PBS targets CUL3 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CUL3 fusion protein Ag1555
• Full Name: cullin 3
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 80-89 kDa
• GenBank Accession Number: BC039598
• Gene Symbol: CUL3
• Gene ID (NCBI): 8452
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q13618
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.