Home
Products
Primary Antibodies
CUL4A/CUL4B Polyclonal antibody (12916-1-AP)

Featured Product
KD/KO Validated

CUL4A/CUL4B Polyclonal antibody

CUL4A/CUL4B Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 12916-1-AP

Publications(99)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

CUL4B, cullin 4B, Cullin-4B, KIAA0695

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIPIF/ICC

WB analysis using 12916-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12916-1-AP (CUL4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa cells using 12916-1-AP
WB result of CUL4B antibody (12916-1-AP, 1:2,000) with si-Control and si-CUL4B transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 12916-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12916-1-AP (CUL4A/CUL4B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 12916-1-AP
IP result of anti-CUL4B (IP:12916-1-AP, 3ug; Detection:12916-1-AP 1:1000) with HeLa cells lysate 4650ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 12916-1-AP
IP result of anti-CUL4A/CUL4B (IP:12916-1-AP, 4ug; Detection:12916-1-AP 1:5000) with HeLa cells lysate 1280 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 12916-1-AP
IP result of anti-CUL4B (IP:12916-1-AP, 4ug; Detection:12916-1-AP 1:5000) with HeLa cells lysate 1280 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 12916-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (12916-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 12916-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (12916-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 12916-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (12916-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (99)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HT-29 cells
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12916-1-AP targets CUL4A/CUL4B in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3563 Product name: Recombinant human CUL4B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC036216 Sequence: MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGIL Predict reactive species
Full Name	cullin 4B
Calculated Molecular Weight	913 aa, 104 kDa
Observed Molecular Weight	80, 102 kDa
GenBank Accession Number	BC036216
Gene Symbol	CUL4B
Gene ID (NCBI)	8450
RRID	AB_2086699
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13620
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). This antibody may detect both CUL4A (87 kDa) and CUL4B(104 kDa).

Protocols

Product Specific Protocols
IF protocol for CUL4A/CUL4B antibody 12916-1-AP	Download protocol
IP protocol for CUL4A/CUL4B antibody 12916-1-AP	Download protocol
WB protocol for CUL4A/CUL4B antibody 12916-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Signal Transduct Target Ther A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer Authors - Tiantian Xu View Article
human	WB	Signal Transduct Target Ther The CRL3BTBD9 E3 ubiquitin ligase complex targets TNFAIP1 for degradation to suppress cancer cell migration. Authors - Lihui Li View Article
human	WB	Nature Targeted protein degradation via intramolecular bivalent glues Authors - Oliver Hsia KD Validated View Article
mouse	WB	Cancer Cell Nuclear cyclin D1/CDK4 kinase regulates CUL4 expression and triggers neoplastic growth via activation of the PRMT5 methyltransferase. Authors - Aggarwal Priya P View Article
human	WB	Nat Med Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity. Authors - Ruth Eichner KD Validated View Article
human	IHC,IF	Science CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins. Authors - Chao Yu View Article