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- KD/KO Validated
CUL4B Polyclonal antibody
CUL4B Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 12916-1-AP
|Positive WB detected in||HEK-293 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
12916-1-AP targets CUL4B in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CUL4B fusion protein Ag3563|
|Full Name||cullin 4B|
|Calculated molecular weight||913 aa, 104 kDa|
|Observed molecular weight||102 kDa|
|GenBank accession number||BC036216|
|Gene ID (NCBI)||8450|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).
FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.
Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.
Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity.
Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.
Nuclear cyclin D1/CDK4 kinase regulates CUL4 expression and triggers neoplastic growth via activation of the PRMT5 methyltransferase.