CUL4B Polyclonal antibody

• Featured Product
• KD/KO Validated

CUL4B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Publications

3

Cat no : 20882-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis using 20882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa cells using 20882-1-AP

  WB result of CUL4B antibody (20882-1-AP, 1:500) with si-Control and si-CUL4B transfected HeLa cells.

• 

  WB analysis of Y79 using 20882-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 20882-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20882-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20882-1-AP

  IP Result of anti-CUL4B (IP:20882-1-AP, 4ug; Detection:20882-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human tonsillitis using 20882-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 20882-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 20882-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 20882-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 20882-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20882-1-AP (CUL4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 20882-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20882-1-AP (CUL4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20882-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20882-1-AP (CUL4B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20882-1-AP

  Immunofluorescent analysis of Hela cells, using CUL4B antibody 20882-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 20882-1-AP

  Immunofluorescent analysis of HepG2 cells, using CUL4B antibody 20882-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse thymus tissue, HeLa cells, Jurkat cells, SH-SY5Y cells, Y79 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human tonsillitis tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

The immunogen of 20882-1-AP is CUL4B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CUL4B fusion protein Ag15024
• Full Name: cullin 4B
• Calculated molecular weight: 913 aa, 104 kDa
• Observed molecular weight: 102 kDa
• GenBank accession number: BC036216
• Gene symbol: CUL4B
• Gene ID (NCBI): 8450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). Proteintech's CUL4B antibody 20882-1-AP can specifically recognize CUL4B.

Publications

Species	Application	Title
	IP	Neuropsychopharmacology; Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus.; Authors - Gonca Bayraktar ; KD Validated
mouse	IP	Front Immunol; PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway.; Authors - Nianyin Lv
mouse	IF	Cells; Cullin4 E3 Ubiquitin Ligases Regulate Male Gonocyte Migration, Proliferation and Blood-Testis Barrier Homeostasis.; Authors - Yan Yin

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