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CUL4B Polyclonal antibody

CUL4B Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 20882-1-AP



Tested Applications

Positive WB detected inmouse thymus tissue, Jurkat cells, Y79 cells, SH-SY5Y cells, HeLa cells, rat thymus tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman tonsillitis tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20882-1-AP targets CUL4B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL4B fusion protein Ag15024
Full Name cullin 4B
Calculated Molecular Weight 913 aa, 104 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC036216
Gene Symbol CUL4B
Gene ID (NCBI) 8450
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). Proteintech's CUL4B antibody 20882-1-AP can specifically recognize CUL4B.


Product Specific Protocols
WB protocol for CUL4B antibody 20882-1-APDownload protocol
IHC protocol for CUL4B antibody 20882-1-APDownload protocol
IF protocol for CUL4B antibody 20882-1-APDownload protocol
IP protocol for CUL4B antibody 20882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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