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Cullin 5 Monoclonal antibody

Cullin 5 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

ELISA

Conjugate

Unconjugated

CloneNo.

4A3E9

Cat no : 60093-1-Ig

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using Cullin 5 Monoclonal antibody (60093-1-Ig)

    WB analysis of Y79 using 60093-1-Ig

    Y79 cells were subjected to SDS PAGE followed by western blot with 60093-1-Ig (Cullin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using Cullin 5 Monoclonal antibody (60093-1-Ig)

    IHC staining of human skin using 60093-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin slide using 60093-1-Ig (Cullin 5 antibody at dilution of 1:50 (under 40x lens).

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60093-1-Ig targets Cullin 5 in ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cullin 5 fusion protein Ag5453
Full Name cullin 5
Calculated Molecular Weight 91 kDa
GenBank Accession NumberBC063306
Gene Symbol CUL5
Gene ID (NCBI) 8065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocols

Product Specific Protocols
WB protocol for Cullin 5 antibody 60093-1-IgDownload protocol
IHC protocol for Cullin 5 antibody 60093-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols