Cullin 5 Monoclonal antibody

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  WB analysis of Y79 using 60093-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 60093-1-Ig (Cullin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 60093-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin slide using 60093-1-Ig (Cullin 5 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 60093-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin slide using 60093-1-Ig (Cullin 5 antibody at dilution of 1:50 (under 40x lens).

Cullin 5 Monoclonal Antibody for ELISA

Cat No. 60093-1-Ig

Clone No.4A3E9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

ELISA

Synonyms

Cullin 5

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Product Information

60093-1-Ig targets Cullin 5 in ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5453

  Product name: Recombinant human CUL5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 435-780 aa of BC063306

  Sequence: KEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA

• Full Name: cullin 5
• Calculated Molecular Weight: 91 kDa
• GenBank Accession Number: BC063306
• Gene Symbol: Cullin 5
• Gene ID (NCBI): 8065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q93034
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.