Cullin 5 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 83869-1-RR (same clone as 83869-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83869-1-RR (CUL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83869-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U-2 OS using 83869-1-RR (same clone as 83869-1-PBS)

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug Cullin 5 Recombinant antibody (83869-1-RR, Clone:240737A12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83869-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 83869-1-RR (same clone as 83869-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cullin 5 Recombinant antibody (83869-1-RR, Clone:240737A12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83869-1-PBS in a different storage buffer formulation.

Cullin 5 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83869-1-PBS

Clone No.240737A12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

CUL5, 240737A12, CUL 5, CUL-5, Cullin-5

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Product Information

83869-1-PBS targets Cullin 5 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34620

  Product name: Recombinant human CUL5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC063306

  Sequence: MATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLM

• Full Name: cullin 5
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC063306
• Gene Symbol: Cullin 5
• Gene ID (NCBI): 8065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q93034
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The cullin family proteins are scaffold proteins for the RING finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. In humans, there are eight members of cullins, including CUL1, 2, 3, 4A, 4B, 5, 7, and CUL9 with an evolutionarily conserved cullin homology domain at the C-terminus, responsible for their interaction with the RING protein, assembling 8 cullin-based RING dependent E3 ubiquitin ligases to target thousands of substrates for ubiquitination and degradation (PMID: 32334051). Cullin 5 is the key scaffolding protein of Cullin RING ligase-5 (CRL-5). Cullin 5 plays positive roles in regulating cell growth, proliferation and physiological and other processes in the human body (PMID: 32194910). Cullin 5 has been detected in the form of double bands in the results of Western blot, which might be caused by neddylation (PMID: 36449095; 31294695).