CWC15 Recombinant monoclonal antibody

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• CWC15 Recombinant monoclonal antibody (87890-1-RR)

• 

  WB analysis using 87890-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87890-1-RR (CWC15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 87890-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CWC15 antibody (87890-1-RR, Clone: 260028B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CWC15 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87890-1-RR

Clone No.260028B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

AD-002, C11orf5, Cwf15, HSPC148, ORF5

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Tested Applications

• Positive WB detected in: SW 1990 cells, PC-3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87890-1-RR targets CWC15 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag18116

  Product name: Recombinant human CWC15 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-229 aa of BC040946

  Sequence: MTTAARPTFEPARGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYRQTTQDAPEEVRNRDFRRELEERERAAAREKNRDRPTREHTTSSSVSKKPRLDQIPAANLDADDPLTDEEDEDFEEESDDDDTAALLAELEKIKKERAEEQARKEQEQKAEEERIRMENILSGNPLLNLTGPSQPQANFKVKRRWDDDVVFKNCAKGVDDQKKDKRFVNDTLRSEFHKKFMEKYIK

• Full Name: CWC15 spliceosome-associated protein homolog (S. cerevisiae)
• Calculated Molecular Weight: 229 aa, 27 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC040946
• Gene Symbol: CWC15
• Gene ID (NCBI): 51503
• RRID: AB_3745809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9P013
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CWC15, also named as C11orf5 or Spliceosome-associated protein CWC15 homolog, is a 229 amino acid protein, which belongs to the CWC15 family. CWC15 as a Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.