CWF19L2 Polyclonal antibody

CWF19L2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24872-1-AP

Synonyms

CWF19 like protein 2, CWF19L2

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Validation Data Gallery

• 

  WB analysis of HeLa using 24872-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24872-1-AP (CWF19L2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24872-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24872-1-AP (CWF19L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 24872-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24872-1-AP (CWF19L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 24872-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24872-1-AP (CWF19L2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 24872-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24872-1-AP (CWF19L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24872-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24872-1-AP (CWF19L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, PC-3 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24872-1-AP is CWF19L2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CWF19L2 fusion protein Ag21738
• Full Name: CWF19-like 2, cell cycle control (S. pombe)
• Calculated Molecular Weight: 894 aa, 104 kDa
• Observed Molecular Weight: 103 kDa
• GenBank Accession Number: BC110442
• Gene Symbol: CWF19L2
• Gene ID (NCBI): 143884
• RRID: AB_2879769
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.