CX3CL1 Recombinant antibody

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  WB analysis using 83738-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83738-5-RR (Cx3cl1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 83738-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83738-5-RR (CX3CL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83738-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83738-5-RR (CX3CL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83738-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83738-5-RR against Mouse CX3CL1 were performed. The affinity constant is 0.744 nM.

CX3CL1 {ptg:Brand} Recombinant Antibody for WB, IHC, ELISA

Cat No. 83738-5-RR

Clone No.240738E2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

Scyd1, Cxc3, CX3C, ABCD 3, 240738E2

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse lung tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83738-5-RR targets CX3CL1 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: chemokine (C-X3-C motif) ligand 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 55-75 kDa
• GenBank Accession Number: NM_009142.3
• Gene Symbol: Cx3cl1
• Gene ID (NCBI): 20312
• RRID: AB_3671337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O35188
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CX3CL1, also known as fractalkine, is a trans-membrane chemokine that acts as a ligand for both CX3CR1 and integrins αvβ3 and α4β1 (PMID: 9479488; 23125415). CX3CL1 is expressed by many hematopoietic and nonhematopoietic cells, including macrophages, endothelial cells, neurons, and fibroblasts. It is involved in immune responses and inflammation (PMID: 21045240). CX3CL1 exists in a membrane-anchored molecule and a soluble form, generated by proteolytic cleavage (PMID: 11495925; 12714508).