CX3CL1 Recombinant monoclonal antibody, PBS Only

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• CX3CL1 Recombinant monoclonal antibody, PBS Only (85353-2-PBS)

CX3CL1 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85353-2-PBS

Clone No.251665G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

A-152E5.2, ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3C membrane-anchored chemokine

• 

  WB analysis using 85353-2-RR (same clone as 85353-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85353-2-RR (CX3CL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85353-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 85353-2-RR (same clone as 85353-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85353-2-RR (CX3CL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85353-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 85353-2-RR (same clone as 85353-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85353-2-RR (CX3CL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85353-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

85353-2-PBS targets CX3CL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6637

  Product name: recombinant human CX3CL1 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 25-341 aa of BC016164

  Sequence: QHHGVTKCNITCSKMTSKIPVALLIHYQQNQASCGKRAIILETRQHRLFCADPKEQWVKDAMQHLDRQAAALTRNGGTFEKQIGEVKPRTTPAAGGMDESVVLEPEATGESSSLEPTPSSQEAQRALGTSPELPTGVTGSSGTRLPPTPKAQDGGPVGTELFRVPPVSTAATWQSSAPHQPGPSLWAEAKTSEAPSTQDPSTQASTASSPAPEENAPSEGQRVWGQGQSPRPENSLEREEMGPVPAHTDAFQDWGPGSMAHVSVVPVSSEGTPSREPVASGSWTPKAEEPIHATMDPQRLGVLITPVPDAQAATRRQ

• Full Name: chemokine (C-X3-C motif) ligand 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 55-75 kDa
• GenBank Accession Number: BC016164
• Gene Symbol: CX3CL1
• Gene ID (NCBI): 6376
• RRID: AB_3744158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78423
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CX3CL1, also known as fractalkine, is a trans-membrane chemokine that acts as a ligand for both CX3CR1 and integrins αvβ3 and α4β1 (PMID: 9479488; 23125415). CX3CL1 is expressed by many hematopoietic and nonhematopoietic cells, including macrophages, endothelial cells, neurons, and fibroblasts. It is involved in immune responses and inflammation (PMID: 21045240). CX3CL1 exists in a membrane-anchored molecule and a soluble form, generated by proteolytic cleavage (PMID: 11495925; 12714508).