CX3CR1 Recombinant monoclonal antibody

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• CX3CR1 Recombinant monoclonal antibody (82183-2-RR)

CX3CR1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 82183-2-RR

Clone No.251201C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Beta chemokine receptor like 1, Beta chemokine receptor-like 1, C X3 C CKR 1, CCRL1, CMK BRL 1

• 

  WB analysis using 82183-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82183-2-RR (CX3CR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of SH-SY5Y using 82183-2-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CX3CR1 antibody (82183-2-RR, Clone: 251201C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, rat brain tissue, mouse brain tissue, THP-1 cells, HEK-293 cells, HeLa cells
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82183-2-RR targets CX3CR1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: chemokine (C-X3-C motif) receptor 1
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC028078
• Gene Symbol: CX3CR1
• Gene ID (NCBI): 1524
• RRID: AB_3743402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49238
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12). The two CX3CR1 bands exhibited can be attributed to various factors (PMID: 30628679).