CXADR Polyclonal antibody

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• CXADR Polyclonal antibody (11777-1-AP)

CXADR Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11777-1-AP Copied!

Publications (2)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CAR, Coxsackievirus B-adenovirus receptor, CVB3 binding protein, CVB3-binding protein, HCAR

• 

  WB analysis using 11777-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11777-1-AP (CXADR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 11777-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11777-1-AP (CXADR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 11777-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11777-1-AP (CXADR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Caco-2 cells, BxPC-3 cells, HeLa cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11777-1-AP targets CXADR in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2369

  Product name: Recombinant human CXADR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-365 aa of BC003684

  Sequence: MALLLCFVLLCGVVDFARSLSITTPEEMIEKAKGETAYLPCKFTLSPEDQGPLDIEWLISPADNQKVDQVIILYSGDKIYDDYYPDLKGRVHFTSNDLKSGDASINVTNLQLSDIGTYQCKVKKAPGVANKKIHLVVLVKPSGARCYVDGSEEIGSDFKIKCEPKEGSLPLQYEWQKLSDSQKMPTSWLAEMTSSVISVKNASSEYSGTYSCTVRNRVGSDQCLLRLNVVPPSNKAGLIAGAIIGTLLALALIGLIIFCCRKKRREEKYEKEVHHDIREDVPPPKSRTSTARSYIGSNHSSLGSMSPSNMEGYSKTQYNQVPSEDFERTPQSPTLPPAKVAAPNLSRMGAIPVMIPAQSKDGSIV

• Full Name: coxsackie virus and adenovirus receptor
• Calculated Molecular Weight: 365 aa, 40 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC003684
• Gene Symbol: CXADR
• Gene ID (NCBI): 1525
• RRID: AB_2087443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78310
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coxsackievirus and adenovirus receptor (CXADR) is a member of junctional adhesion molecules, which are glycosylated transmembrane proteins that belong to the Ig superfamily (PMID: 17916321). CXADR plays essential roles in blastocyst formation, including cavitation and the proper subcellular localization of tight junction proteins into the apical edge of the outer blastomeres (PMID: 26729920). It is also involved in the transepithelial migration of leukocytes through adhesive interactions with junctional adhesion molecule-like protein (JAML) (PMID: 15800062).

Publications

What published studies show

The CXADR Polyclonal antibody (catalog number 11777-1-AP) has 2 total citations. These appear in Advanced Science and Oncology Research. The associated research fields include pancreatic ductal adenocarcinoma, where vitamin D binding protein and integrin β1 promote perineural invasion, and tumor immunotherapy, involving oncolytic adenovirus H101 enhancing anti-tumor effects of PD-1 blockade via CD47 downregulation.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Adv Sci (Weinh); Vitamin D Binding Protein, a Ligand of Integrin beta 1, Motivates Both Tumor Cells and Schwann Cells to Promote Perineural Invasion in Pancreatic Ductal Adenocarcinoma; Authors - Shan Zhang
	WB	Oncol Res; Oncolytic adenovirus H101 enhances the anti-tumor effects of PD-1 blockade via CD47 downregulation in tumor cells; Authors - Chenxiao Qiao

Reviews

1.0 out of 5

1 verified customer reviews

What customers say

The single review for this product reports use in immunohistochemistry on zebrafish retina at a 1:100 dilution. The reviewer assigned a 1-star rating and provided no written comments. Overall, the limited feedback suggests the product may not have performed well for this specific application, though the absence of detailed comments makes it difficult to identify the underlying issue.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina