CXCL4/PF4 Recombinant antibody

• 

  WB analysis of mouse serum using 85647-1-RR

  mouse serum was subjected to SDS PAGE followed by western blot with 85647-1-RR (CXCL4/PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 85647-1-RR

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 85647-1-RR (CXCL4/PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CXCL4/PF4 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85647-1-RR

Clone No.243087F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CXCL4, PF4, SCYB4, C X C motif chemokine 4, C-X-C motif chemokine 4

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Tested Applications

• Positive WB detected in: mouse serum, human peripheral blood platelets

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85647-1-RR targets CXCL4/PF4 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FusionProtein
• Full Name: platelet factor 4
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: NM_002619.4
• Gene Symbol: PF4
• Gene ID (NCBI): 5196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02776
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation