CXCL8/IL-8 Monoclonal antibody

IL8/CXCL8 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Publications

8

CloneNo.

3C4E2

Cat no : 60141-1-Ig

Synonyms

GCP/IL 8 protein IV, GCP/IL 8 protein V, GCP/IL 8 protein VI, GCP1

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Validation Data Gallery

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  WB analysis of Recombinant protein using 60141-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60141-1-Ig (IL8/CXCL8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 60141-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 60141-1-Ig(IL8/CXCL8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 60141-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 60141-1-Ig(IL8/CXCL8 antibody) at dilution of 1:50 (under 40x lens).

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 4 publications below

Product Information

60141-1-Ig targets IL8/CXCL8 in WB, IHC,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL8/CXCL8 fusion protein Ag10552
• Full Name: interleukin 8
• Calculated Molecular Weight: 99 aa, 11 kDa
• GenBank Accession Number: BC013615
• Gene Symbol: IL8
• Gene ID (NCBI): 3576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human		Nat Commun; Synergistic IL-6 and IL-8 paracrine signalling pathway infers a strategy to inhibit tumour cell migration.; Authors - Hasini Jayatilaka
human	IHC	Cancers (Basel); Secreted Frizzled-Related Protein 1 as a Biomarker against Incomplete Age-Related Lobular Involution and Microcalcifications' Development.; Authors - Alisson Clemenceau
human	WB	Lab Chip; A simple multi-well stretching device to induce inflammatory responses of vascular endothelial cells.; Authors - Jiasheng Wang
human	WB	J Hematol Oncol; DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis.; Authors - Qian Liu
human	IHC	Placenta; Placental inflammation by HMGB1 activation of TLR4 at the syncytium.; Authors - Line H Tangerås
human	IHC	Cytokine; Association between physical activity and the expression of mediators of inflammation in normal breast tissue among premenopausal and postmenopausal women.; Authors - Mirette Hanna