CXCL8,IL8 Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG2a

Reactivity

Capture: Human

Detection: Human

Applications

Cytometric bead array, Sandwich ELISA

Range

Sandwich ELISA: 0.049-3.125 ng/mL

Cat No : MP50089-1

Print datasheet

Synonyms

GCP/IL 8 protein I, IL 8(1 77), (Ser IL 8)72, GCP/IL 8 protein VI, GCP/IL 8 protein V, T cell chemotactic factor, CXCL8, GCP/IL 8 protein II, LUCT, IL 8, IL8, NAF, LECT, MONAP, GCP1, GCP/IL 8 protein III, LYNAP, MDNCF, C X C motif chemokine 8, Emoctakin, interleukin 8, NAP1, GCP 1, GCP/IL 8 protein IV, (Ala IL 8)77, Neutrophil activating factor

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Validation Data Gallery

• 

  Standard curve of MP50089-1

  Sandwich ELISA standard curve of MP50089-1, CXCL8,IL8 Monoclonal Matched Antibody Pair - BSA and Azide free. Capture antibody: 68744-1-PBS. Detection antibody: 60141-2-PBS. Standard:HZ-1318. Range: 0.049-3.125 ng/mL.

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Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP50089-1 targets CXCL8,IL8 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.049-3.125 ng/mL (Sandwich ELISA);

Capture antibody: CXCL8,IL8 Monoclonal antibody, PBS Only (Capture) 68744-1-PBS (1A6C6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: CXCL8/IL-8 Monoclonal antibody, PBS Only (Detector) 60141-2-PBS (3D11D5). 100 μg. Concentration 1 mgl/ml.

Matched pair protein standard: HZ-1318

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

• Capture antibody
• Detection antibody

• Catalog Number: 68744-1-PBS
• Tested Reactivity: Human
• Immunogen: CXCL8,IL8 fusion protein HZ-1318
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Full Name: interleukin 8
• Synonyms: CXCL8, Emoctakin, GCP 1, GCP1, IL 8, IL8, interleukin 8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, Neutrophil activating factor, T cell chemotactic factor
• GenBank accession number: BC013615
• Gene symbol: IL8
• Uniprot: P10145
• Gene ID (NCBI): 3576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

• Catalog Number: 60141-2-PBS
• Tested Reactivity: Human
• Immunogen: CXCL8/IL-8 fusion protein Ag10552
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Full Name: interleukin 8
• Synonyms: C X C motif chemokine 8, CXCL8, CXCL8/IL-8, Emoctakin, GCP 1, GCP1, IL8, IL-8, interleukin 8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, Neutrophil activating factor, T cell chemotactic factor
• GenBank accession number: BC013615
• Gene symbol: IL8
• Uniprot: P10145
• Gene ID (NCBI): 3576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.