CXCR6 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 84591-1-RR (same clone as 84591-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84591-1-RR (CXCR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84591-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84591-1-RR (same clone as 84591-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84591-1-RR (CXCR6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84591-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 84591-1-RR (same clone as 84591-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CXCR6 antibody (84591-1-RR, Clone: 241662E5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84591-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 84591-1-RR (same clone as 84591-1-PBS)

  Immunofluorescent analysis of un-fixed human lung cancer tissue using CXCR6 antibody (84591-1-RR, Clone: 241662E5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 84591-1-PBS in a different storage buffer formulation.

CXCR6 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 84591-1-PBS

Clone No.241662E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

241662E5, BONZO, CD186, CDw186, CXC R6

---

Product Information

84591-1-PBS targets CXCR6 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: chemokine (C-X-C motif) receptor 6
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC033584
• Gene Symbol: CXCR6
• Gene ID (NCBI): 10663
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O00574
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CXCR6, or C-X-C chemokine receptor 6, is a G protein-coupled receptor with seven transmembrane domains that belong to the CXC chemokine receptor family. This family also includes CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, and CXCR7. CXCR6 is expressed in several T lymphocyte subsets and bone marrow stromal cells. The encoded protein and its exclusive ligand, chemokine ligand 16 (CCL16), are part of a signaling pathway that regulates T lymphocyte migration to various peripheral tissues such as the liver, spleen red pulp, intestine, lungs, and skin. CXCR6 plays a crucial role in immunosurveillance through its interaction with epithelial cells and certain subpopulations of intratumor dendritic cells (DC), particularly DC3, which express CXCL16 at the membrane.